Document Detail


In vitro study on blocking mTOR signaling pathway in EGFR-TKI resistance NSCLC.
MedLine Citation:
PMID:  25063068     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the effect and mechanism of inhibitor everolimus on EGFR-TKI resistance NSCLC.
METHODS: MTT assay was used to detect proliferation of human non-small cell lung cancer cell line A549. Flow cytometry was used to detect the changes of apoptosis and cycle distribution in each group after 24 h and 48 h. RT-PCR was used to detect the changes of PTEN and 4EBP1 expression levels after 48 h of monotherapy and combination therapy.
RESULTS: MTT assay showed that everolimus had dose-dependent inhibition against growth of A549 cells. Flow cytometry showed when everolimus could induce apoptosis and induce G0/G1 phase cell cycle arrest, which was time-dependent (P<0.05). RT-PCR showed everolimus could increase PTEN and 4EBP1 expression.
CONCLUSIONS: mTOR inhibitor everolimus has an inhibitory effect on EGFR-TKI resistant NSCLC, which cannot reverse the resistance effect of EGFR-TKI resistant cell line A549. The relationship between EGFR/AKT signaling pathway and the mTOR signaling pathway and the mechanism in non-small cell lung cancer need further study.
Authors:
Xu-Dong Xiang; Jing Yu; Gao-Feng Li; Lin Xie; Heng Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  7     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-07-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  394-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2014 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
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