| In vitro stimulation of penetration gland emptying by Trichobilharzia szidati and T. regenti (Schistosomatidae) cercariae. Quantitative collection and partial characterization of the products. | |
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MedLine Citation:
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PMID: 15868186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Induction of penetration gland emptying by cercariae of the bird schistosomes Trichobilharzia szidati and T. regenti employing linoleic acid, linolenic acid, praziquantel and calcium ionophore A23187 showed that both postacetabular and circumacetabular cells released their content at chosen stimulant concentrations. The gland secretions consisted of soluble and insoluble parts. The former one adhering to the ground seemed to have different saccharide composition from the glands of Schistosoma mansoni. It bound labelled saccharides, thus exhibiting lectin-like activity. Protein profiles of the latter one were identical after stimulation by all four stimulants in T. szidati. The soluble secretions contained several proteolytic enzymes; 31 kDa and 33 kDa cysteine proteases were identified in E/S products of T. szidati and T. regenti, respectively. The circumacetabular glands contained a significant amount of calcium. Immunohistochemistry revealed that the origin of E/S products after in vitro stimulation is in both penetration glands and tegumental structures. No crossreactivity was observed between the bird schistosomes and a serum raised against S. mansoni elastase. |
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Authors:
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Libor Mikes; Lenka Zìdková; Martin Kasný; Jan Dvorák; Petr Horák |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-05-03 |
Journal Detail:
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Title: Parasitology research Volume: 96 ISSN: 0932-0113 ISO Abbreviation: Parasitol. Res. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-07-01 Completed Date: 2005-10-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 230-41 Citation Subset: IM |
Affiliation:
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Department of Parasitology, Charles University, Vinicná 7, 12844, Prague 2, Czech Republic, mikes@natur.cuni.cz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anthelmintics / pharmacology Calcimycin / pharmacology Dose-Response Relationship, Drug Exocrine Glands / drug effects, secretion* Helminth Proteins / chemistry, metabolism* Host-Parasite Interactions / physiology Linoleic Acids / pharmacology Linolenic Acids / pharmacology Mice Mice, Inbred BALB C Praziquantel / pharmacology Schistosomatidae / drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anthelmintics; 0/Helminth Proteins; 0/Linoleic Acids; 0/Linolenic Acids; 52665-69-7/Calcimycin; 55268-74-1/Praziquantel |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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