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In vitro silencing of myostatin gene by shrnas in chicken embryonic myoblast cells.
MedLine Citation:
PMID:  23292805     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
RNA interference (RNAi) represents one of the potential mechanisms of regulation of gene expression. Selective down regulation of Myostatin (MSTN), a member of transforming growth factor-β (TGF-β) super family and a negative regulator of myogenesis, has been demonstrated to enhance skeletal muscle growth. In the present study, we studied shRNA induced myostatin gene silencing in chicken embryonic myoblast cells using seven different shRNA expressing constructs by Reverse transcription quantitative Real Time PCR (RT-qPCR). Myostatin silencing efficiency of all shRNA constructs were first evaluated in Human embryonic kidney cell line 293T (HEK293T) cells, where we observed 30 to 75.6% reduction in myostatin expression, followed by chicken embryo myoblast cells which revealed up to 55% reduction in myostatin expression along with up regulation of MyoD by 4.65-folds. Consistent with the earlier observations, the transfection of cells with plasmids led to significant increase in interferon responsive genes OAS1 and IFN β (2 to 112 folds), independent of myostatin silencing in both HEK293T and chicken embryonic myoblast cells. Our study suggests that apart from shRNA sequences, cell type specific factors may play significant role in determining the knockdown efficiency of shRNAs. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 2013.
Authors:
Ajai K Tripathi; Mansi K Aparnathi; Amrutlal K Patel; Chaitanya G Joshi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-7
Journal Detail:
Title:  Biotechnology progress     Volume:  -     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 American Institute of Chemical Engineers (AIChE).
Affiliation:
Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India, PIN- 388001.
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