Document Detail


In-vitro shoot regeneration via caulogenesis in fern, Pteris vittata L.
MedLine Citation:
PMID:  23359991     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
An attempt was made to standardize the protocol for the shoot regeneration via caulogenesis in Pteris vittata L. employing leaf primordium explants. Calli were induced on Murashige and Skoog's (MS) and Parkers and Thompson's (P and T) media supplemented with different combinations of 2, 4-dichlorophenoxyaceticacid (2, 4-D), 6-benzylaminopurine (BAP), alpha-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA). A combination of full strength MS medium with 2, 4-D (2.26 microM) and BAP (2.22 microM) was found to be ideal for profuse callusing (80%) against other combinations. More shoot differentiation (2.8 +/- 0.06) was achieved from the calli on one-fourth strength of P and T media fortified with BAP (4.44 microM) and NAA (2.68 microM) when compared to other combinations but statistically not significant.
Authors:
Shastri P Shukla; P B Khare
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of environmental biology / Academy of Environmental Biology, India     Volume:  33     ISSN:  0254-8704     ISO Abbreviation:  J Environ Biol     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2013-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8300544     Medline TA:  J Environ Biol     Country:  India    
Other Details:
Languages:  eng     Pagination:  683-7     Citation Subset:  IM    
Affiliation:
Department of Plant Sciences, University of Hyderabad, Hyderabad--500 046, India. sp_shukla2002@yahoo.co.in
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