| In-vitro shoot regeneration via caulogenesis in fern, Pteris vittata L. | |
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MedLine Citation:
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PMID: 23359991 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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An attempt was made to standardize the protocol for the shoot regeneration via caulogenesis in Pteris vittata L. employing leaf primordium explants. Calli were induced on Murashige and Skoog's (MS) and Parkers and Thompson's (P and T) media supplemented with different combinations of 2, 4-dichlorophenoxyaceticacid (2, 4-D), 6-benzylaminopurine (BAP), alpha-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA). A combination of full strength MS medium with 2, 4-D (2.26 microM) and BAP (2.22 microM) was found to be ideal for profuse callusing (80%) against other combinations. More shoot differentiation (2.8 +/- 0.06) was achieved from the calli on one-fourth strength of P and T media fortified with BAP (4.44 microM) and NAA (2.68 microM) when compared to other combinations but statistically not significant. |
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Authors:
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Shastri P Shukla; P B Khare |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of environmental biology / Academy of Environmental Biology, India Volume: 33 ISSN: 0254-8704 ISO Abbreviation: J Environ Biol Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2013-01-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8300544 Medline TA: J Environ Biol Country: India |
Other Details:
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Languages: eng Pagination: 683-7 Citation Subset: IM |
Affiliation:
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Department of Plant Sciences, University of Hyderabad, Hyderabad--500 046, India. sp_shukla2002@yahoo.co.in |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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