Document Detail


In vitro sensitivity and resistance of cultured human squamous carcinoma cells to cis-platinum and methotrexate.
MedLine Citation:
PMID:  6438583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine whether resistance to chemotherapy in advanced head and neck squamous cell carcinoma stems from biochemical mechanisms and to assess the potential usefulness of new anticarcinogens, an in vitro test would be highly desirable. In the past 4 years our laboratory has developed methodology to establish squamous carcinoma cell lines in tissue culture from patients with squamous cancer of the head and neck. We used some of these lines to compare the in vitro effects of methotrexate on squamous carcinoma, fibrosarcoma, and melanoma cells. Three of the squamous carcinoma lines were tested for sensitivity to both methotrexate and cis-platinum; all but one squamous carcinoma cell line exposed to methotrexate showed growth inhibition at the physiologically attainable concentration of 10(-7) M. In contrast, a tenfold higher concentration was required to produce similar effects in the melanoma, while no concentration inhibited fibrosarcoma growth. Cis-platinum showed consistent squamous carcinoma cytotoxicity in 10(-5) M and 10(-6) M concentrations. This approach should be useful for determining the relative sensitivity and resistance of head and neck cancers to chemotherapeutic agents in vitro.
Authors:
J Spiegel; T E Carey; S Shimoura; C J Krause
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery     Volume:  92     ISSN:  0194-5998     ISO Abbreviation:  Otolaryngol Head Neck Surg     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-01-03     Completed Date:  1985-01-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8508176     Medline TA:  Otolaryngol Head Neck Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  524-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / pathology*
Cell Count
Cell Line
Cell Survival / drug effects
Cisplatin / pharmacology*
Colony-Forming Units Assay*
Fibrosarcoma / pathology
Head and Neck Neoplasms / pathology*
Humans
Melanoma / pathology
Methotrexate / pharmacology*
Tumor Stem Cell Assay*
Grant Support
ID/Acronym/Agency:
NCI CA00621/CA/NCI NIH HHS; NCI CA28564/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
15663-27-1/Cisplatin; 59-05-2/Methotrexate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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