Document Detail

In vitro senescence enhances IL-6 production in human gingival fibroblasts induced by lipopolysaccharide from Campylobacter rectus.
MedLine Citation:
PMID:  8735906     Owner:  NLM     Status:  MEDLINE    
The production of interleukin-6 (IL-6) in human gingival fibroblasts (Gin cells) is increased by lipopolysaccharide (LPS) from Campylobacter rectus (C. rectus), which is associated with adult periodontitis; however, the age-related changes in the susceptibility of Gin cells to C. rectus LPS remain unclear. We examined the influence of in vitro senescence on C. rectus LPS-stimulated IL-6 production in Gin cells. LPS was prepared from C. rectus ATCC 33238 using hot phenol-water. The Gin cells were established from healthy gingival tissue removed from three patients, aged 10-12 years. The cells were cultured until confluence then stimulated with LPS (0.01, 0.1, 1.0 and 10.0 micrograms/ml). Levels of IL-6 released in the medium were measured after incubation for 3, 6, 9, 12, and 24 h. In both young (5-6 population doublings) and senescent (17-20 population doublings) cells, LPS stimulated IL-6 production in a dose- and time-dependent manner. In response to 0.01-10.0 micrograms/ml of LPS, IL-6 production in the senescent cells was higher than that in the young cells. Using cells from each of the three donors, we found that this phenomenon of higher LPS-stimulated IL-6 production in senescent cells was reproducible. The greater capacity of the senescent cells to synthesize IL-6 in response to LPS was a higher production of mRNA for IL-6. This increase of IL-6 production induced by C. rectus LPS in senescent Gin cells could help to explain the increased susceptibility to periodontal diseases shown by aged individuals.
N Ogura; U Matsuda; F Tanaka; Y Shibata; H Takiguchi; Y Abiko
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  87     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-11-06     Completed Date:  1996-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  47-59     Citation Subset:  IM    
Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
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MeSH Terms
Base Sequence
Campylobacter / pathogenicity
Cell Aging / immunology*
Fibroblasts / cytology,  immunology
Gene Expression
Gingiva / cytology,  immunology*
Interleukin-6 / biosynthesis*,  genetics
Lipopolysaccharides / isolation & purification,  pharmacology
Periodontitis / etiology
RNA, Messenger / genetics
Reg. No./Substance:
0/Interleukin-6; 0/Lipopolysaccharides; 0/RNA, Messenger

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