Document Detail


In vitro selection for Fusarium wilt resistance in Gladiolus.
MedLine Citation:
PMID:  18713429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cormels pieces of four Fusarium susceptible Gladiolus cultivars (Friendship, Peter Pears, Victor Borge and Novalux) formed friable calli when cultured in vitro on Murashige and Skoog basal medium containing various concentrations of auxin and cytokinin. The friable calli established cell suspensions. Plantlet regeneration was obtained from the control callus, control cell suspension derived callus and in vitro selected Fusarium oxysporum Schlecht. resistant cell-lines of Friendship. The in vitro cormlets showed 85-95% germination after breaking dormancy of 8 weeks at 4 degrees C. Cell suspensions of all four Gladiolus cultivars were found to be highly sensitive to fusaric acid. Gradual increase in fusaric acid concentrations to the cell-suspension cultures decreased cell growth considerably. One albino plant was found from the second generation of the in vitro selected cell line of Friendship. The albino plant was found to be highly susceptible to F. oxysporum. The cormlets of all in vitro selected cell lines of Friendship were inoculated with a conidial suspension of the F. oxysporum before planting and were also sprayed with the same spore suspension for further characterization when the height of plants was about 6 cm. The four selected cell lines showed the same response whether or not they were inoculated with conidia of the F. oxysporum. Plantlets of all of the selected cell lines exhibited significant growth as compared with the control after application of conidia of the F. oxysporum.
Authors:
Idrees Ahmad Nasir; Sheikh Riazuddin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of integrative plant biology     Volume:  50     ISSN:  1744-7909     ISO Abbreviation:  J Integr Plant Biol     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-08-20     Completed Date:  2008-10-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101250502     Medline TA:  J Integr Plant Biol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  601-12     Citation Subset:  IM    
Affiliation:
National Centre of Excellence in Molecular Biology, University of the Punjab, Canal Bank, Thokar Niaz Baig, Lahore 53700, Pakistan. dr.idrees@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques
Cell Size / drug effects
Cell Survival / drug effects
Culture Media
Fusaric Acid / pharmacology
Fusarium / drug effects,  physiology*
Immunity, Innate / drug effects
Iridaceae / cytology,  growth & development*,  immunology,  microbiology*
Plant Diseases / immunology*,  microbiology*
Regeneration / drug effects
Selection, Genetic*
Chemical
Reg. No./Substance:
0/Culture Media; 536-69-6/Fusaric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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