| In vitro secretion of digestive phospholipase A(2) by midguts isolated from tobacco hornworms, manduca sexta | |
| | |
MedLine Citation:
|
PMID: 10536046 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We report on secretion of phospholipase A(2) (PLA(2)) by in vitro preparations of midguts isolated from tobacco hornworms, Manduca sexta. This enzyme is responsible for hydrolysis of fatty acids from the sn-2 position of phospholipids, a necessary step in fatty acid absorption. The in vitro midgut preparations are competent to secrete PLA(2) into incubation buffer. Secretion began within the first 30 min of incubation and increased to a maximum at 8 h. We selected 2 h incubations because substantial loss of tissue integrity was observed after 8 h incubations. Using 2 h incubations, we recorded increased secretion of digestive PLA(2) from midguts incubated in buffer amended with diet or with yeast as a component of the diet. We also recorded small increases in secretion of PLA(2) from midguts incubated in buffer amended with a specific phospholipid, phosphatidylcholine. Midguts incubated in buffer amended with increased concentrations of phospholipid did not yield higher levels of PLA(2) activity. Lepidopteran midguts can be divided into three regions, and we recorded the highest secretion of PLA(2) from the middle region and lowest secretion from the anterior region. Because isolated midguts responded to food chemicals with increased secretion of digestive PLA(2), we suggest that secretion of digestive enzymes in tobacco hornworms is regulated by a prandial and/or paracrine mechanism, as suggested for digestive proteases in other insect species. Arch. Insect Biochem. Physiol. 42:179-187, 1999.Copyright 1999 Wiley-Liss, Inc. |
| | |
Authors:
|
Rana; Stanley |
Related Documents
:
|
1529266 - Relative importance of pancreatic, hepatic, and mucosal bicarbonate in duodenal neutral... 11137176 - Chronic effects of different fatty acids and leptin in ins-1 cells. 3011456 - Leukotriene effects upon the transgastric potential difference and pepsin secretion. 2480636 - Effect of the proton pump inhibitor, nc-1300, on gastric mucus secretion in rats. 3464946 - Human liver apolipoprotein b-100 cdna: complete nucleic acid and derived amino acid seq... 18546426 - Studies on scenedesmus acutus growth ii. effect of autotrophic and mixotrophic growth o... |
Publication Detail:
|
Type: JOURNAL ARTICLE |
Journal Detail:
|
Title: Archives of insect biochemistry and physiology Volume: 42 ISSN: 1520-6327 ISO Abbreviation: Arch. Insect Biochem. Physiol. Publication Date: 1999 Nov |
Date Detail:
|
Created Date: 1999-11-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8501752 Medline TA: Arch Insect Biochem Physiol Country: UNITED STATES |
Other Details:
|
Languages: Eng Pagination: 179-87 Citation Subset: - |
Affiliation:
|
Insect Biochemical Physiology Laboratory, University of Nebraska, Lincoln, Nebraska. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Proregion of Bombyx mori cysteine proteinase functions as an intramolecular chaperone to promote pro...
Next Document: Relationships among food and contact signals in experimental group-living young of tegenaria atrica