| In vitro screening of potential anti-cancer chemicals: effect of purine pyrimidine analogues on seed germination. | |
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MedLine Citation:
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PMID: 7292509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Germinating Cicer arietinum seeds were used to simulate embryonic tissue for evaluating toxicity of purine and pyrimidine analogues. 6-Mercaptopurine stimulated germination, whereas 8-azaadenine, 8-azaguanine, 2,6-diaminopurine, 6-amino, 2-hydroxypurine and 5-fluorouracil inhibited germination. 6-Methyluracil, 5-aminouracil and methylxanthines, viz. caffeine, theophylline, theobromine and uric acid exhibited little inhibitory effects. RNA synthesis ensued immediately after water imbibition and reached maximum at 12 h of germination. 2,6-Diaminopurine, 8-azaadenine, 8-azaguanine and 5-fluorouracil inhibited RNA synthesis. Cyclic-AMP, adenosine, indole-3-acetic acid (IAA) and folic acid partially reversed the inhibitory effects produced by purine pyrimidine analogues. Several hitherto untested pyrimidine analogues inhibited germination as well as RNA synthesis. Inhibition of Escherichia coli dihydrofolate reductase was used for purposes of comparison. |
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Authors:
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C M Dwivedi; H Junjappa; C R Krishna Murti |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Toxicology Volume: 21 ISSN: 0300-483X ISO Abbreviation: Toxicology Publication Date: 1981 |
Date Detail:
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Created Date: 1981-12-21 Completed Date: 1981-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0361055 Medline TA: Toxicology Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 251-60 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents* Drug Evaluation, Preclinical / methods Plants / drug effects*, growth & development Purines / pharmacology* Pyrimidines / pharmacology* Structure-Activity Relationship Tetrahydrofolate Dehydrogenase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Purines; 0/Pyrimidines; EC 1.5.1.3/Tetrahydrofolate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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