Document Detail


In vitro safety of intravitreal moxifloxacin for endophthalmitis treatment.
MedLine Citation:
PMID:  18299076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate the safety of moxifloxacin for intravitreal application in a cell culture model. SETTING: Department of Ophthalmology, Ludwig-Maximilians-Universität, Munich, Germany. METHODS: Primary human retinal pigment epithelium (RPE) cells, ARPE19 cells, and primary optic nerve head astrocyte (ONHA) cells were treated with concentrations of moxifloxacin ranging from 10 to 750 microg/mL. Possible toxic effects and median inhibitory concentration were evaluated after 24 hours as well as under conditions of oxidative stress. After treating the RPE and ONHA cell lines with tumor necrosis factor-alpha (TNF-alpha; 10 microg/mL), lipopolysaccharides (LPS; 20 microg/mL), and interleukin-6 (IL-6; 20 microg/mL), the effects of moxifloxacin on cellular viability under conditions of inflammation were investigated. Toxicity was evaluated by measuring the inhibition of RPE cell proliferation with the tetrazolium dye-reduction assay (MTT; 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide). Cell viability was quantified by a microscopic live/dead assay. RESULTS: At concentrations higher than 150 microg/mL, moxifloxacin had adverse effects on primary RPE, ARPE19, and ONHA cell proliferation and viability. Lower concentrations did not affect RPE or ONHA cell proliferation and viability when administered for 24 hours. No significant decrease in proliferation and viability was observed after preincubation with TNF-alpha, LPS, and IL-6 for 24 hours and subsequent treatment with moxifloxacin concentrations of 10 to 150 microg/mL for 24 hours. Hydrogen peroxide exposure did not increase cellular toxicity. CONCLUSIONS: No significant toxicity of moxifloxacin was seen on primary RPE cells, ARPE19 cells, or ONHA cells at concentrations up to 150 microg/mL. Intravitreal use of moxifloxacin up to this concentration may be safe and effective for the treatment of endophthalmitis.
Authors:
Marcus Kernt; Aljoscha Steffen Neubauer; Michael W Ulbig; Anselm Kampik; Ulrich Welge-Lüssen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cataract and refractive surgery     Volume:  34     ISSN:  0886-3350     ISO Abbreviation:  J Cataract Refract Surg     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-26     Completed Date:  2008-04-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8604171     Medline TA:  J Cataract Refract Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  480-8     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Ludwig-Maximilians-Universität, Munich, Germany. marcus.kernt@med.uni-muenchen.de
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MeSH Terms
Descriptor/Qualifier:
Anti-Infective Agents / toxicity*
Astrocytes / drug effects*
Aza Compounds / toxicity*
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Endophthalmitis / drug therapy*
Humans
Interleukin-6 / pharmacology
Lipopolysaccharides / pharmacology
Optic Disk / cytology*
Oxidative Stress
Pigment Epithelium of Eye / drug effects*
Quinolines / toxicity*
Tumor Necrosis Factor-alpha / pharmacology
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Aza Compounds; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Quinolines; 0/Tumor Necrosis Factor-alpha; 0/moxifloxacin

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