Document Detail

In vitro response of encapsulated somatic embryos of Lagerstroemia indica L.
MedLine Citation:
PMID:  15991582     Owner:  NLM     Status:  MEDLINE    
A method to produce encapsulatable units for synthetic seeds was developed in L. indica. Somatic embryos were harvested from leaf derived embryogenic callus on Murashige and Skoog's basal medium supplemented with 2, 4-dichlorophenoxy acetic acid (2, 4-D, 0.5 mg/l), 6-benzyl amino purine (BAP, 1 mg/l) and ascorbic acid (AA, 50 mg/l). The embryos were encapsulated in alginate beads and dehydrated. Germination ability of the artificial seeds were investigated. The frequency of regeneration from the encapsulated embryos was significantly affected by (i) the concentration of alginate (ii) the duration of storage, and (iii) the effect of different types of media. A 2% sodium alginate concentration on MS salts resulted in significantly higher germination frequencies than at other concentrations. L. indica showed maximum germination on MS medium (93.84%) after 6 weeks of culture. The germinated synthetic seeds with well developed roots and shoots were transferred successfully to green house. This is the first report on artificial seeds in Lagerstroemnia indica.
M H Niranjan; M S Sudarshana
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of experimental biology     Volume:  43     ISSN:  0019-5189     ISO Abbreviation:  Indian J. Exp. Biol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-04     Completed Date:  2005-08-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0233411     Medline TA:  Indian J Exp Biol     Country:  India    
Other Details:
Languages:  eng     Pagination:  552-4     Citation Subset:  IM    
Plant Tissue Culture Lab, PG Department of Botany, University of Mysore, Manasagangotri, Mysore 570 006, India.
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MeSH Terms
2,4-Dichlorophenoxyacetic Acid / chemistry
Adenine / analogs & derivatives*,  chemistry
Alginates / chemistry
Ascorbic Acid / chemistry
Calcium Chloride / metabolism
Culture Media / pharmacology
Culture Techniques
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Lagerstroemia / embryology*,  genetics,  physiology*
Plant Bark / metabolism
Plant Growth Regulators / chemistry
Plant Leaves / metabolism*
Seeds / chemistry,  metabolism*
Time Factors
Reg. No./Substance:
0/Alginates; 0/Culture Media; 0/Hexuronic Acids; 0/Plant Growth Regulators; 10043-52-4/Calcium Chloride; 1214-39-7/N-benzyladenine; 50-81-7/Ascorbic Acid; 525-79-1/Kinetin; 576-37-4/Glucuronic Acid; 73-24-5/Adenine; 9005-32-7/alginic acid; 94-75-7/2,4-Dichlorophenoxyacetic Acid

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