| In vitro release of vascular endothelial growth factor from gadolinium-doped biodegradable microspheres. | |
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MedLine Citation:
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PMID: 15170848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A drug delivery vehicle was constructed that could be visualized noninvasively with MRI. The biodegradable polymer poly(DL-lactic-co-glycolic acid) (PLGA) was used to fabricate microspheres containing vascular endothelial growth factor (VEGF) and the MRI contrast agent gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). The microspheres were characterized in terms of size, drug and contrast agent encapsulation, and degradation rate. The PLGA microspheres had a mean diameter of 48 +/- 18 microm. The gadolinium loading was 17 +/- 3 microg/mg polymer and the VEGF loading was 163 +/- 22 ng/mg polymer. Electron microscopy revealed that the Gd was dispersed throughout the microspheres and it was confirmed that the Gd loading was sufficient to visualize the microspheres under MRI. VEGF and Gd-DTPA were released from the microspheres in vitro over a period of approximately 6 weeks in three phases: a burst, followed by a slow steady-state, then a rapid steady-state. Biodegradable Gd-doped microspheres can be effectively used to deliver drugs in a sustained manner, while being monitored noninvasively with MRI. |
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Authors:
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Anthony Z Faranesh; Monet T Nastley; Cristina Perez de la Cruz; Michael F Haller; Patrice Laquerriere; Kam W Leong; Elliot R McVeigh |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 51 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-01 Completed Date: 2004-09-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 1265-71 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, Maryland, USA. faranesh@nih.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biodegradation, Environmental Cell Division / drug effects Cell Line Contrast Media Drug Carriers* Gadolinium DTPA Humans Infusion Pumps, Implantable* Lactic Acid* Microspheres* Polyglycolic Acid* Polymers* Vascular Endothelial Growth Factor A / pharmacokinetics*, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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Z01 HL004608-08/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Drug Carriers; 0/Polymers; 0/Vascular Endothelial Growth Factor A; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 80529-93-7/Gadolinium DTPA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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