Document Detail

In vitro release of ACTH from dispersed rat pars intermedia cells. III. Multiple forms of ACTH biological activity.
MedLine Citation:
PMID:  215929     Owner:  NLM     Status:  MEDLINE    
We have reported that both an extract of rat hypothalamus-stalk-median eminence (HE) and 5-hydroxytryptamine (5-HT) will stimulate the release of ACTH biological activity from acutely, non-enzymically dispersed rat pars intermedia (PI) cells. We have also found that the ACTH activity within the PI cells is composed of 4 chromatographically separable entities, but only a small proportion of the total ACTH activity co-elutes with ACTH1-39. In the present study we subjected the media, after incubation of PI cells with either HE or 5-HT, to sequential chromatography on Sephadex G-50 F and G-100 SF. We found the same 4 ACTH-like moieties as in the PI cell extracts. Only the release of the 2 earliest eluting components, presumably of much larger molecular dimensions than ACTH1-39, was enhanced by either HE or 5-HT. There was no augmented release of the ACTH component having molecular dimensions similar to ACTH1-39. Thus, in our in vitro system, the ACTH biological activity released from PI cells is not the size of ACTH1-39, but is composed of at least 2 much larger molecules which possess ACTH (as well as some MSH) biological activity.
J Kraicer; N L Elliot; A E Zimmerman
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Neuroendocrinology     Volume:  27     ISSN:  0028-3835     ISO Abbreviation:  Neuroendocrinology     Publication Date:  1978  
Date Detail:
Created Date:  1979-03-13     Completed Date:  1979-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0035665     Medline TA:  Neuroendocrinology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  86-96     Citation Subset:  IM    
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MeSH Terms
Adrenocorticotropic Hormone / isolation & purification,  secretion*
Hypothalamus / physiology
Pituitary Gland, Anterior / drug effects,  secretion*
Serotonin / pharmacology
Tissue Extracts
Reg. No./Substance:
0/Tissue Extracts; 50-67-9/Serotonin; 9002-60-2/Adrenocorticotropic Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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