Document Detail

In vitro reestablishment of cell-cell contacts in adult rat cardiomyocytes. Functional role of transmembrane components in the formation of new intercalated disk-like cell contacts.
MedLine Citation:
PMID:  10352149     Owner:  NLM     Status:  MEDLINE    
Primary adult rat cardiomyocytes (ARC)in culture are shown to be a model system for cardiac cell hypertrophy in vitro. ARC undergo a process of morphological transformation and grow only by increase in cell size, however, without loss of the cardiac phenotype. The isolated cells spread and establish new cell-cell contacts, eventually forming a two-dimensional heart tissue-like synchronously beating cell sheet. The reformation of specific cell contacts (intercalated disks) is shown also between ventricular and atrial cardiomyocytes by using antibodies against the gap junction protein connexin-43 and after microinjection into ARC of N-cadherin cDNA fused to reporter green fluorescent protein (GFP) cDNA. The expressed fusion protein allowed the study of live cell cultures and of the dynamics of the adherens junction protein N-cadherin during the formation of new cell-cell contacts. The possible use of the formed ARC cell-sheet cells under microgravity conditions as a test system for the reformation of the cytoskeleton of heart muscle cells is proposed.
H M Eppenberger; C Zuppinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  13 Suppl     ISSN:  0892-6638     ISO Abbreviation:  FASEB J.     Publication Date:  1999  
Date Detail:
Created Date:  1999-06-21     Completed Date:  1999-06-21     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S83-9     Citation Subset:  IM; S    
Department of Biology, Institute of Cell Biology, Swiss Federal Institute of Technology ETH, CH-8093 Zurich, Switzerland.
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MeSH Terms
Cadherins / genetics
Cell Communication*
Cell Membrane / metabolism
Cells, Cultured
DNA, Complementary
Green Fluorescent Proteins
Luminescent Proteins / genetics
Microscopy, Electron
Models, Biological
Myocardium / cytology*,  metabolism,  ultrastructure
Rats, Sprague-Dawley
Recombinant Fusion Proteins / genetics
Reg. No./Substance:
0/Cadherins; 0/DNA, Complementary; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

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