| In vitro radioisotopic labeling of proteins associated with purified polyoma virions. | |
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MedLine Citation:
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PMID: 4372416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An in vitro procedure using [(14)C]formaldehyde and sodium borohydride was used to label the proteins of purified polyoma virions. This method, which labeled the three capsid proteins and the four internal histones of polyoma virus, did not alter the biological and biophysical characteristics of the virus. In addition to the seven viral proteins, five additional radioactive peaks were resolved on polyacrylamide gels. Four of these peaks could be attributed to growing the virus in the presence of serum. The fifth peak appears to be a nonhistone host-contributed protein made before infection. |
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Authors:
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J McMillen; R A Consigli |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 14 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1974 Dec |
Date Detail:
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Created Date: 1975-02-18 Completed Date: 1975-02-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1627-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Radioisotopes DNA, Viral / analysis Electrophoresis, Polyacrylamide Gel Isotope Labeling / methods* Polyomavirus* Sodium Dodecyl Sulfate Viral Proteins* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/DNA, Viral; 0/Viral Proteins; 151-21-3/Sodium Dodecyl Sulfate |
| Comments/Corrections | |
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