| In vitro protein synthesis by oligodendroglial cells. | |
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MedLine Citation:
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PMID: 19604847 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Oligodendroglial cells were isolated from spinal cord and brain stem tissue from 30-day-old rats according to the method of Poduslo and Norton except that acetylated trypsin at a concentration of 0.05% was used instead of unmodified trypsin, and the density gradient was slightly changed. Isolated oligodendroglial cells were found to incorporate [U-(14)C]leucine into TCA-insoluble protein up to 120 min. This incorporation was inhibited by cycloheximide and chloramphenicol. Purity of the cell preparation was assessed by phase contrast microscopy and by assay of 2',3'-cyclic nucleotide phosphohydrolase activity. |
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Authors:
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N L Banik; M E Smith |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience letters Volume: 2 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1976 Jun |
Date Detail:
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Created Date: 2009-07-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 235-8 Citation Subset: - |
Affiliation:
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Department of Neurology, Veterans Administration Hospital, Palo Alto U.S.A. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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