| In vitro proliferation and differentiation of human mesenchymal stem cells on hydroxyapatite versus human demineralised bone matrix with and without osteogenic protein-1. | |
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MedLine Citation:
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PMID: 19063689 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Allografting is currently used in lower limb reconstruction surgery. Demineralised bone matrix (DBM) is more osteoinductive compared with allografts but lacks mechanical strength. Osteogenic protein-1 (OP-1) can improve the osteoinductivity of the allograft, however recent reports indicate significant allograft resorption when it is combined with OP-1. OBJECTIVES: Our hypothesis was that hydroxyapatite (HA) with human-mesenchymal stem cells (h-MSCs) and OP-1 (HA+h-MSCs+OP-1) has similar osteoinductive properties to human-DBM+h-MSCs. The objective was to evaluate h-MSC proliferation (by tritiated thymidine incorporation, total DNA Hoechst 33258 and scanning electron microscopy) and osteogenic differentiation (from alkaline phosphatase activity) in human demineralised bone matrix (h-DBM) and HA, with or without OP-1. RESULTS: H-MSC proliferation on HA+OP-1 was significantly higher compared with that on HA at all time points (p < 0.05) and compared with DBM alone [day 1, (198.4 vs 95.4) p = 0.042; day 14 (286.1 vs 119.9), p < 0.001]. H-MSC proliferation was higher in DBM+OP-1, at all time points compared with HA+OP-1 but the difference was not statistically significant (p > 0.05). H-MSC differentiation was significantly higher in HA+OP-1 compared with HA (p < 0.05) but not significantly different from diffferentiation on DBM alone (p > 0.05). Differentiation was significantly higher on DBM+OP-1 at all time points compared with HA (p < 0.05) and with HA+OP-1 [day 1, (21.1 vs 10.1) (p = 0.03); day 7 (39.4 vs 7.1) (p < 0.01); day 14 (40.2 vs 14.4) (p < 0.001)]. CONCLUSIONS: When HA+h-MSCs is combined with OP-1 in vitro its osteogenic potential is similar to that of DBM+h-MSCs alone which may be adequate for non-weight-bearing applications. Mechanical testing however is of great importance for weight-bearing applications and the in vivo testing of the composite graft HA+h-MSCs+OP-1 vs DBM+h-MSCs is recommended. |
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Authors:
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Eleftherios Tsiridis; Zubier Ali; Amit Bhalla; Zakareya Gamie; Manolis Heliotis; Neelam Gurav; Sanjukta Deb; Lucy DiSilvio |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Expert opinion on biological therapy Volume: 9 ISSN: 1744-7682 ISO Abbreviation: Expert Opin Biol Ther Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-09 Completed Date: 2009-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101125414 Medline TA: Expert Opin Biol Ther Country: England |
Other Details:
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Languages: eng Pagination: 9-19 Citation Subset: IM |
Affiliation:
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Academic Unit of Trauma and Orthopaedic Surgery, Clarendon Wing, Leeds General Infirmary, Leeds, UK. etsiridis@doctors.org.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Alkaline Phosphatase / metabolism Bone Demineralization Technique Bone Matrix / cytology* Bone Morphogenetic Protein 7 / pharmacology* Cell Differentiation Cell Proliferation Cells, Cultured Durapatite / pharmacology* Humans Male Mesenchymal Stem Cells / cytology* Microscopy, Electron, Scanning |
| Chemical | |
Reg. No./Substance:
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0/Bone Morphogenetic Protein 7; 1306-06-5/Durapatite; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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