Document Detail

In vitro production of lysine from 2,2'-diaminopimelic acid by rumen protozoa.
MedLine Citation:
PMID:  1787428     Owner:  NLM     Status:  MEDLINE    
Rumen protozoa can produce lysine from free 2,2'-diaminopimelic acid (DAP). However, the quantitative importance of this transformation has been disputed; lysine contents of protozoal incubation supernatants reported by Onodera & Kandatsu and Masson & Ling show a 26-fold difference. The in vitro experimental methods of both groups were compared to determine the causes of this difference. Lysine production was proportional to DAP concentration. Results with rumen protozoa from sheep or goats were similar. The incubation medium and deproteinizing procedure of the Welsh group gave a two-fold increase in lysine production compared with Japanese protocols. Omissions of rice starch from protozoal incubations slightly increased lysine production, whereas omissions of antibacterial agents resulted in varying, yet relatively small changes. The greatest cause of the difference was the number of rumen protozoa incubated. When this factor was taken into account, the difference in the maximum rates of lysine production between the Welsh and Japanese groups was only three-fold, namely 4.5 versus 15.0 nmol lysine/10(5) protozoa/h. Adding other amino acids to the incubations suggested that DAP uptake by rumen protozoa may occur via transport system ASC. The importance of DAP metabolism by protozoa as a source of lysine for ruminant host animals is discussed.
R Onodera; H Takashima; J R Ling
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of protozoology     Volume:  38     ISSN:  0022-3921     ISO Abbreviation:  J. Protozool.     Publication Date:    1991 Jul-Aug
Date Detail:
Created Date:  1992-03-25     Completed Date:  1992-03-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985197R     Medline TA:  J Protozool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  421-5     Citation Subset:  IM    
Laboratory of Animal Nutrition and Biochemistry, Faculty of Agriculture, Miyazaki University, Japan.
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MeSH Terms
Amino Acids
Anti-Infective Agents
Culture Media
Diaminopimelic Acid / metabolism*
Eukaryota / metabolism*
Goats / parasitology
Rumen / parasitology*
Salicylic Acids
Sheep / parasitology
Reg. No./Substance:
0/Amino Acids; 0/Anti-Infective Agents; 0/Culture Media; 0/Picrates; 0/Salicylic Acids; 583-93-7/Diaminopimelic Acid; 88-89-1/picric acid; 9005-25-8/Starch; 97-05-2/sulfosalicylic acid

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