Document Detail


In vitro production of lysine from 2,2'-diaminopimelic acid by rumen protozoa.
MedLine Citation:
PMID:  1787428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rumen protozoa can produce lysine from free 2,2'-diaminopimelic acid (DAP). However, the quantitative importance of this transformation has been disputed; lysine contents of protozoal incubation supernatants reported by Onodera & Kandatsu and Masson & Ling show a 26-fold difference. The in vitro experimental methods of both groups were compared to determine the causes of this difference. Lysine production was proportional to DAP concentration. Results with rumen protozoa from sheep or goats were similar. The incubation medium and deproteinizing procedure of the Welsh group gave a two-fold increase in lysine production compared with Japanese protocols. Omissions of rice starch from protozoal incubations slightly increased lysine production, whereas omissions of antibacterial agents resulted in varying, yet relatively small changes. The greatest cause of the difference was the number of rumen protozoa incubated. When this factor was taken into account, the difference in the maximum rates of lysine production between the Welsh and Japanese groups was only three-fold, namely 4.5 versus 15.0 nmol lysine/10(5) protozoa/h. Adding other amino acids to the incubations suggested that DAP uptake by rumen protozoa may occur via transport system ASC. The importance of DAP metabolism by protozoa as a source of lysine for ruminant host animals is discussed.
Authors:
R Onodera; H Takashima; J R Ling
Related Documents :
11177198 - Effect of underfeeding on metabolism of portal-drained viscera in ewes.
12501348 - Synthesis of phenylalanine and production of other related compounds from phenylpyruvic...
7319938 - Effects of primary, secondary and tertiary amines on in vitro cellulose digestion and v...
7076598 - Liver arginase activity and urea metabolism in beef heifers fed urea diets and abomasal...
12473978 - Burning mouth syndrome (bms): an open trial of comparative efficacy of alpha-lipoic aci...
23372838 - Serum uric acid levels and cerebral microbleeds in patients with acute ischemic stroke.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of protozoology     Volume:  38     ISSN:  0022-3921     ISO Abbreviation:  J. Protozool.     Publication Date:    1991 Jul-Aug
Date Detail:
Created Date:  1992-03-25     Completed Date:  1992-03-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985197R     Medline TA:  J Protozool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  421-5     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Nutrition and Biochemistry, Faculty of Agriculture, Miyazaki University, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids
Animals
Anti-Infective Agents
Culture Media
Diaminopimelic Acid / metabolism*
Eukaryota / metabolism*
Goats / parasitology
Picrates
Rumen / parasitology*
Salicylic Acids
Sheep / parasitology
Starch
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Anti-Infective Agents; 0/Culture Media; 0/Picrates; 0/Salicylic Acids; 583-93-7/Diaminopimelic Acid; 88-89-1/picric acid; 9005-25-8/Starch; 97-05-2/sulfosalicylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Malaria sporozoites release circumsporozoite protein from their apical end and translocate it along ...
Next Document:  Glucan synthesis in Pneumocystis carinii.