| In vitro production of llama (Lama glama) embryos by IVF and ICSI with fresh semen. | |
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MedLine Citation:
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PMID: 18054452 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interest for South American camelids has increased in the last years. The aim of the present research was to compare the in vitro production of Lama glama embryos using two techniques: in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen samples were obtained by electroejaculation and incubated at 38 degrees C in a 25% (v/v) collagenase solution. The cleavage and embryo development rates were compared between the different experimental groups. Only the number of cleaved oocytes was less when ICSI with no activation was used (p<0.05). |
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Authors:
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Pamela A Conde; Carolina Herrera; Virginia L Trasorras; Susana M Giuliano; Ariel Director; Marcelo H Miragaya; María G Chaves; María I Sarchi; Diana Stivale; Carlos Quintans; Alicia Agüero; Bruno Rutter; Sergio Pasqualini |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-23 |
Journal Detail:
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Title: Animal reproduction science Volume: 109 ISSN: 1873-2232 ISO Abbreviation: Anim. Reprod. Sci. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-10-30 Completed Date: 2008-12-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7807205 Medline TA: Anim Reprod Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 298-308 Citation Subset: IM |
Affiliation:
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Halitus Biotecnología, Halitus Instituto Medico, Capital Federal, Argentina. pamela.conde@halitus.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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analogs & derivatives,
pharmacology Animals Antioxidants / pharmacology Argentina Camelids, New World / physiology* Ejaculation Electric Stimulation Embryo, Mammalian / physiology* Female Fertilization in Vitro / methods*, veterinary Heparin / pharmacology Ionomycin / pharmacology Male Oocyte Retrieval Penicillamine / pharmacology Pregnancy Protein Kinase Inhibitors / pharmacology Semen / physiology* Sperm Injections, Intracytoplasmic / methods*, veterinary Taurine / analogs & derivatives, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Protein Kinase Inhibitors; 107-35-7/Taurine; 300-84-5/hypotaurine; 52-67-5/Penicillamine; 56092-81-0/Ionomycin; 73-24-5/Adenine; 9005-49-6/Heparin; 938-55-6/N(6),N(6)-dimethyladenine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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