Document Detail


In vitro precultivation alleviates post-implantation inflammation and enhances development of tissue-engineered tubular cartilage.
MedLine Citation:
PMID:  19258698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue-engineered tubular cartilage is a promising graft for tracheal reconstruction. But polylactic acid/polyglycolic acid (PLA/PGA) fibers, the frequently used scaffolds for cartilage engineering, often elicit an obvious inflammation response following implantation into immunocompetent animals. We propose that the inflammation could be alleviated by in vitro precultivation. In this study, after in vitro culture for either 2 days (direct implantation group (DI)) or for 2 weeks (precultivation implantation group (PI)), autologous tubular chondrocyte-PLA/PGA constructs were subcutaneously implanted into rabbits. In the PI group, after 2 weeks of precultivation, most of the fibers were found to be completely embedded in an extracellular matrix (ECM) produced by the chondrocytes. Importantly, no obvious inflammatory reaction was observed after in vivo implantation and homogeneous cartilage-like tissue was formed with biomechanical properties close to native tracheal cartilage at 4 weeks post-implantation. In the DI group, however, an obvious inflammatory reaction was observed within and around the cell-scaffold constructs at 1 week implantation and only sporadic cartilage islands separated by fibrous tissue were observed at 4 weeks. These results demonstrated that the post-implantation inflammatory reaction could be alleviated by in vitro precultivation, which contributes to the formation of satisfactory tubular cartilage for tracheal reconstruction.
Authors:
Xusong Luo; Guangdong Zhou; Wei Liu; Wen Jie Zhang; Lian Cen; Lei Cui; Yilin Cao
Related Documents :
17573168 - Indentation stiffness does not discriminate between normal and degraded articular carti...
19258698 - In vitro precultivation alleviates post-implantation inflammation and enhances developm...
16705708 - Effect of oral diacerein (dar) in an experimental hip chondrolysis model.
10190258 - Detection of experimentally produced occult microfractures at the bone-cartilage interf...
23852228 - Assessment of the impact strength of the denture base resin polymerized by various proc...
19149068 - The effect of location and number of endosseous implants on retention and stability of ...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-04
Journal Detail:
Title:  Biomedical materials (Bristol, England)     Volume:  4     ISSN:  1748-605X     ISO Abbreviation:  Biomed Mater     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-07     Completed Date:  2009-08-13     Revised Date:  2010-03-11    
Medline Journal Info:
Nlm Unique ID:  101285195     Medline TA:  Biomed Mater     Country:  England    
Other Details:
Languages:  eng     Pagination:  025006     Citation Subset:  IM    
Affiliation:
Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Absorbable Implants
Animals
Biocompatible Materials
Biomechanics
Cartilage / cytology*,  pathology
Cartilage, Articular / cytology,  pathology
Cell Culture Techniques*
Chondrocytes / metabolism
Extracellular Matrix / metabolism
Inflammation
Lactic Acid / chemistry
Polyglycolic Acid / chemistry
Polymers / chemistry
Rabbits
Tissue Engineering / methods*
Trachea / pathology*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers; 26009-03-0/Polyglycolic Acid; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Study on the enhanced cellular uptake effect of daunorubicin on leukemia cells mediated via function...
Next Document:  Aqueous synthesis of gold nanoparticles and their cytotoxicity in human dermal fibroblasts-fetal.