Document Detail

In vitro precultivation alleviates post-implantation inflammation and enhances development of tissue-engineered tubular cartilage.
MedLine Citation:
PMID:  19258698     Owner:  NLM     Status:  MEDLINE    
Tissue-engineered tubular cartilage is a promising graft for tracheal reconstruction. But polylactic acid/polyglycolic acid (PLA/PGA) fibers, the frequently used scaffolds for cartilage engineering, often elicit an obvious inflammation response following implantation into immunocompetent animals. We propose that the inflammation could be alleviated by in vitro precultivation. In this study, after in vitro culture for either 2 days (direct implantation group (DI)) or for 2 weeks (precultivation implantation group (PI)), autologous tubular chondrocyte-PLA/PGA constructs were subcutaneously implanted into rabbits. In the PI group, after 2 weeks of precultivation, most of the fibers were found to be completely embedded in an extracellular matrix (ECM) produced by the chondrocytes. Importantly, no obvious inflammatory reaction was observed after in vivo implantation and homogeneous cartilage-like tissue was formed with biomechanical properties close to native tracheal cartilage at 4 weeks post-implantation. In the DI group, however, an obvious inflammatory reaction was observed within and around the cell-scaffold constructs at 1 week implantation and only sporadic cartilage islands separated by fibrous tissue were observed at 4 weeks. These results demonstrated that the post-implantation inflammatory reaction could be alleviated by in vitro precultivation, which contributes to the formation of satisfactory tubular cartilage for tracheal reconstruction.
Xusong Luo; Guangdong Zhou; Wei Liu; Wen Jie Zhang; Lian Cen; Lei Cui; Yilin Cao
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-04
Journal Detail:
Title:  Biomedical materials (Bristol, England)     Volume:  4     ISSN:  1748-605X     ISO Abbreviation:  Biomed Mater     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-07     Completed Date:  2009-08-13     Revised Date:  2010-03-11    
Medline Journal Info:
Nlm Unique ID:  101285195     Medline TA:  Biomed Mater     Country:  England    
Other Details:
Languages:  eng     Pagination:  025006     Citation Subset:  IM    
Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
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MeSH Terms
Absorbable Implants
Biocompatible Materials
Cartilage / cytology*,  pathology
Cartilage, Articular / cytology,  pathology
Cell Culture Techniques*
Chondrocytes / metabolism
Extracellular Matrix / metabolism
Lactic Acid / chemistry
Polyglycolic Acid / chemistry
Polymers / chemistry
Tissue Engineering / methods*
Trachea / pathology*
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers; 26009-03-0/Polyglycolic Acid; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid

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