Document Detail


In vitro pre-exposure of Haemonchus contortus L3 to blood eosinophils reduces their establishment potential in sheep.
MedLine Citation:
PMID:  17682295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Different authors have reported that eosinophils are capable of immobilising infective larvae of different species of nematodes in vitro. However, classifying larvae as mobile or immobile is so subjective that it does not always mean all apparently immobile larvae are dead or those that are mobile are capable of surviving further immune responses if administered to their natural hosts. The objective of this experimental study was therefore to substantiate the role of eosinophils in the killing of Haemonchus contortus infective larvae by comparing the infectivity in sheep of larvae that had been incubated with eosinophil-enriched cell suspensions with control larvae. Since it was not possible to isolate pure eosinophils from sheep blood, we were compelled to evaluate the effects of other blood cells contaminating our eosinophil-enriched suspensions. Although eosinophils and neutrophils were the only cells found adherent to H. contortus infective larvae in vitro, induced eosinophils in the presence of immune serum were primarily responsible for the drastic reduction in larval motility compared to the minor effects of neutrophils and mononuclear cells. Corresponding reductions in faecal egg count and worm numbers were observed when the incubated larvae were transferred intra-abomasally to sheep. Interestingly, the proportion of larvae that failed to establish was much higher following incubation with induced eosinophils compared with other cells or with immune serum alone. Although this study did not address the in vivo role of eosinophils in sheep, the results strongly indicate that sheep blood eosinophils have a larval killing potential in vitro, and a larval mobility test alone may not fully explain the level of damage inflicted on the larvae.
Authors:
Getachew Terefe; Christelle Grisez; Françoise Prevot; Jean-Paul Bergeaud; Philippe Dorchies; Jean-Claude Brunel; Dominique François; Isabelle Fourquaux; Philippe Jacquiet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary research     Volume:  38     ISSN:  0928-4249     ISO Abbreviation:  Vet. Res.     Publication Date:    2007 Jul-Aug
Date Detail:
Created Date:  2007-08-07     Completed Date:  2007-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309551     Medline TA:  Vet Res     Country:  France    
Other Details:
Languages:  eng     Pagination:  647-54     Citation Subset:  IM    
Affiliation:
Addis Ababa University, Faculty of Veterinary Medicine, Debre Zeit, PO Box 34, Ethiopia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Eosinophils / parasitology*,  ultrastructure
Haemonchiasis / parasitology,  prevention & control,  veterinary*
Haemonchus / growth & development,  physiology*
Larva / physiology,  ultrastructure
Sheep
Sheep Diseases / parasitology*,  prevention & control*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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