| In vitro potassium transport in the mouse small intestine. | |
| | |
MedLine Citation:
|
PMID: 12617757 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ingested K+ is believed to be absorbed mainly in the small intestine by passive diffusion through the paracellular pathway. To further clarify K+ absorption in the small intestine, we determined the unidirectional flux values of Rb+ in vitro by atomic absorption spectroscopy in the mouse ileum mounted in Ussing chambers under short-circuit conditions. The mucosal-to-serosal Rb+ flux (J(ms)) was larger than the serosal-to-mucosal Rb+ flux (J(sm)), resulting in positive net Rb+ absorption (J(net)). The effect of changing the transmucosal potential (V(t)) showed that J(ms) was composed of both a V(t)-dependent diffusion component and a V(t)-independent non-diffusion component, while J(sm) was composed mainly of a V(t)-dependent component. A forskolin treatment eliminated J(net) mainly due to the increase in J(sm). When animals were fed a low-Na diet, J(net) was mainly eliminated as a result of the increase in J(sm). These findings suggest that K+ is absorbed not only by passive diffusion through the paracellular pathway, but also by an active transport mechanism operating through the cellular pathway. In addition, cAMP and aldosterone may be involved in regulating intestinal K+ transport. |
| | |
Authors:
|
Eiko Inagaki; Koichi Kawamata; Yuichi Suzuki |
Related Documents
:
|
9693207 - Diet supplemented with yoghurt or milk fermented by lactobacillus casei dn-114 001 stim... 13978797 - The absorption of fat by intestine of golden hamster in vitro. 6108077 - Effect of small intestinal bypass on hepatic lipid accumulation in rats. 12711157 - Enhancing effect of chitosan on nasal absorption of salmon calcitonin in rats: comparis... 9785237 - Effect of added dietary fat on performance, rumen characteristics, and plasma metabolit... 9692287 - Obesity wars: a pilot study of very low calorie diets in obese patients in general prac... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Japanese journal of physiology Volume: 52 ISSN: 0021-521X ISO Abbreviation: Jpn. J. Physiol. Publication Date: 2002 Dec |
Date Detail:
|
Created Date: 2003-03-05 Completed Date: 2003-11-20 Revised Date: 2007-03-21 |
Medline Journal Info:
|
Nlm Unique ID: 2985184R Medline TA: Jpn J Physiol Country: Japan |
Other Details:
|
Languages: eng Pagination: 515-20 Citation Subset: IM |
Affiliation:
|
Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, 422-8526 Japan. e-ina@nuas.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adsorption Animals Cell Membrane / drug effects, metabolism* Culture Techniques Forskolin / pharmacology Ileum / drug effects, metabolism* Intestine, Small / metabolism Male Membrane Potentials / drug effects, physiology* Mice Potassium / pharmacokinetics* Rubidium / pharmacokinetics* Sodium / deficiency |
| Chemical | |
Reg. No./Substance:
|
66428-89-5/Forskolin; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: KV2.1 K+ channels underlie major voltage-gated K+ outward current in H9c2 myoblasts.
Next Document: Effects of adrenoceptor antagonists on the cutaneous blood flow increase response to sympathetic ner...