Document Detail

In vitro photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) loaded-poly(lactide-co-glycolide) nanoparticles in LNCaP prostate tumour cells.
MedLine Citation:
PMID:  19070504     Owner:  NLM     Status:  MEDLINE    
In(III)-meso-tetraphenylporphyrin (InTPP) was encapsulated into nanoparticles (smaller than 200 nm) of poly(d,l-lactide-co-glycolide) (PLGA) using the emulsification-evaporation technique. The photodynamic efficacy of InTPP-loaded nanoparticles and its cellular uptake was investigated with LNCaP prostate tumour cells, in comparison with the free InTPP. The effects of incubation time (1-3h), drug concentration (1.8-7.7 micromol/L) and incident light dose (15-45 J/cm(2)) with both encapsulated and free InTPP were studied. The type of cell death induced by the photochemical process using both encapsulated and free InTPP was also investigated. Cell viability was reduced more significantly with increasing values of these effects for InTPP-loaded nanoparticles than with the free drug. The cellular death induced by both encapsulated and free InTPP was preponderantly apoptotic. Confocal laser scanning microscopy data showed that the InTPP-loaded nanoparticles, as well free InTPP, were localized in the cells, and always in the perinuclear region. Encapsulated InTPP was measured by the intensity of fluorescence intensity of cell extracts and was three times more internalized into the cells than was the free InTPP. Electron paramagnetic resonance experiments corroborated the participation of singlet oxygen in the photocytotoxic effect of nanoparticles loaded with InTPP.
André Romero da Silva; Natalia Mayumi Inada; Daniel Rettori; Mariana Ozello Baratti; Anibal Eugenio Vercesi; Renato Atilio Jorge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-13
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  94     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-27     Completed Date:  2009-05-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  101-12     Citation Subset:  IM    
Department of Physical Chemistry, Institute of Chemistry, University of Campinas, Caixa Postal 6154, 13084-862 Campinas-SP, Brazil.
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MeSH Terms
Cell Death / drug effects,  radiation effects
Cell Line, Tumor
Cell Survival / drug effects,  radiation effects
Dimethyl Sulfoxide / pharmacology
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Drug Carriers / chemistry,  pharmacology
Electron Spin Resonance Spectroscopy
Intracellular Space / metabolism
Metalloporphyrins / chemistry*,  metabolism,  pharmacology*,  therapeutic use
Nanoparticles / chemistry*
Particle Size
Photochemical Processes
Photosensitizing Agents / chemistry,  metabolism,  pharmacology,  therapeutic use
Polyglactin 910 / chemistry*,  pharmacology
Prostatic Neoplasms / drug therapy,  pathology*
Singlet Oxygen / metabolism
Surface Properties
Reg. No./Substance:
0/Drug Carriers; 0/Metalloporphyrins; 0/Photosensitizing Agents; 17778-80-2/Singlet Oxygen; 34346-01-5/Polyglactin 910; 67-68-5/Dimethyl Sulfoxide; 72451-33-3/indium tetraphenylporphyrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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