| <i> In vitro </i> perfused human capillary networks. | |
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MedLine Citation:
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PMID: 23320912 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Replicating <i> in vitro </i> the complex <i> in vivo </i> tissue microenvironment has the potential to transform our approach to medicine and also our understanding of biology. In order to accurately model the 3-D arrangement and interaction of cells and extracellular matrix, new microphysiological systems must include a vascular supply. The vasculature not only provides the necessary convective transport of oxygen, nutrients and waste in 3-D culture, it also couples and integrates the responses of organ systems. Here we combine tissue engineering and microfluidic technology to create an in vitro 3-D metabolically active stroma (~ 1 mm<sup>3</sup>) that, for the first time, contains a perfused, living, dynamic, interconnected human capillary network. The range of flow rate (μm/s) and shear rate (1/s) within the network was 0-4000 and 0-1000, respectively, and thus included the normal physiological range. Infusion of FITC dextran demonstrated microvessels (15-50µm) to be largely impermeable to 70 kDa. Our high-throughput biology-directed platform has the potential to impact a broad range of fields that intersect with the microcirculation including tumor metastasis, drug discovery, vascular disease, and environmental chemical toxicity. |
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Authors:
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Monica L Moya; Yu-Hsiang Hsu; Abraham Lee; Christopher C W Hughes; Steven George |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-16 |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: - ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of California, Irvine, Department of Biomedical Engineering, Irvine, California, United States; mmoya@uci.edu. |
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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