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<i> In vitro </i> perfused human capillary networks.
MedLine Citation:
PMID:  23320912     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Replicating <i> in vitro </i> the complex <i> in vivo </i> tissue microenvironment has the potential to transform our approach to medicine and also our understanding of biology. In order to accurately model the 3-D arrangement and interaction of cells and extracellular matrix, new microphysiological systems must include a vascular supply. The vasculature not only provides the necessary convective transport of oxygen, nutrients and waste in 3-D culture, it also couples and integrates the responses of organ systems. Here we combine tissue engineering and microfluidic technology to create an in vitro 3-D metabolically active stroma (~ 1 mm<sup>3</sup>) that, for the first time, contains a perfused, living, dynamic, interconnected human capillary network. The range of flow rate (μm/s) and shear rate (1/s) within the network was 0-4000 and 0-1000, respectively, and thus included the normal physiological range. Infusion of FITC dextran demonstrated microvessels (15-50µm) to be largely impermeable to 70 kDa. Our high-throughput biology-directed platform has the potential to impact a broad range of fields that intersect with the microcirculation including tumor metastasis, drug discovery, vascular disease, and environmental chemical toxicity.  
Authors:
Monica L Moya; Yu-Hsiang Hsu; Abraham Lee; Christopher C W Hughes; Steven George
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  -     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of California, Irvine, Department of Biomedical Engineering, Irvine, California, United States; mmoya@uci.edu.
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