Document Detail


In vitro perfused human capillary networks.
MedLine Citation:
PMID:  23320912     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Replicating in vitro the complex in vivo tissue microenvironment has the potential to transform our approach to medicine and also our understanding of biology. In order to accurately model the 3D arrangement and interaction of cells and extracellular matrix, new microphysiological systems must include a vascular supply. The vasculature not only provides the necessary convective transport of oxygen, nutrients, and waste in 3D culture, but also couples and integrates the responses of organ systems. Here we combine tissue engineering and microfluidic technology to create an in vitro 3D metabolically active stroma (∼1 mm(3)) that, for the first time, contains a perfused, living, dynamic, interconnected human capillary network. The range of flow rate (μm/s) and shear rate (s(-1)) within the network was 0-4000 and 0-1000, respectively, and thus included the normal physiological range. Infusion of FITC dextran demonstrated microvessels (15-50 μm) to be largely impermeable to 70 kDa. Our high-throughput biology-directed platform has the potential to impact a broad range of fields that intersect with the microcirculation, including tumor metastasis, drug discovery, vascular disease, and environmental chemical toxicity.
Authors:
Monica L Moya; Yu-Hsiang Hsu; Abraham P Lee; Christopher C W Hughes; Steven C George
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-02-21
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  19     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-07-23     Completed Date:  2014-07-14     Revised Date:  2014-09-02    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  730-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Capillaries / physiology*
Computer Simulation
Dextrans / metabolism
Dimethylpolysiloxanes / chemistry
Finite Element Analysis
Fluorescein-5-isothiocyanate / analogs & derivatives,  metabolism
Humans
Microcirculation
Microfluidics
Perfusion*
Grant Support
ID/Acronym/Agency:
F32HL105055/HL/NHLBI NIH HHS; P30 CA062203/CA/NCI NIH HHS; P30CA062203/CA/NCI NIH HHS; R01 CA180122/CA/NCI NIH HHS; RC1 ES018361-01/ES/NIEHS NIH HHS; UL1 TR000153/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/fluorescein isothiocyanate dextran; I223NX31W9/Fluorescein-5-isothiocyanate; K3R6ZDH4DU/Dextrans
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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