Document Detail


In vitro performance of an injectable hydrogel/microsphere based immunocyte delivery system for localised anti-tumour activity.
MedLine Citation:
PMID:  19783044     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The current practice of cell immunotherapy against cancer has encountered a substantial challenge, that is, targeted delivery of therapeutic cells to tumour sites is not favourably managed. In this study, we aimed to provide an engineering solution to govern the cell targeting and actions, for which a biomaterial model is developed to mediate the conveyance and accommodation of activated immunocytes with anti-cancer potentials. We fabricated a dual-layered hydrogel/microsphere (GS) composite, which preserves all advantageous features of hydrogel such as injectability and favourable permeability, to achieve genuine localisation and physical immobilisation of the executing immunocytes-macrophages. According to our presented in vitro investigations, the GS immunoconstruct exhibited effective elimination of carcinoma cells as well as high safety free of gene alteration or cell leakage. Notably, unwanted long-term proliferation of the delivered cells was restrained by physical encapsulation in the bio-inert 3D hydrogel frameworks. By these efforts, we have provided an immunocyte delivery platform with which cell-based immunotherapy can be initiated at a desired location and implemented in a controlled manner.
Authors:
Chunming Wang; Gerard N Adrianus; Nan Sheng; Shikai Toh; Yihong Gong; Dong-An Wang
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-26
Journal Detail:
Title:  Biomaterials     Volume:  30     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-10-27     Completed Date:  2010-01-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  6986-95     Citation Subset:  IM    
Affiliation:
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, Singapore 637457, Republic of Singapore.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry,  metabolism
Cell Line, Tumor
Drug Delivery Systems*
Humans
Hydrogels* / chemistry,  metabolism
Immunotherapy / methods*
Injections
Lipopolysaccharides / immunology
Macrophage Activation
Macrophages / cytology,  immunology*
Materials Testing
Microspheres*
Neoplasms / metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Hydrogels; 0/Lipopolysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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