Document Detail

In-vitro ovarian steroidogenesis in women with pelvic congestion.
MedLine Citation:
PMID:  11098029     Owner:  NLM     Status:  MEDLINE    
Follicular fluid steroid content and theca and granulosa cell steroidogenesis in pelvic congestion cystic ovaries were compared with steroidogenic function in both normal and polycystic ovaries. Ovaries were obtained at oophorectomy for benign gynaecological conditions, and classified according to gross morphology at dissection. Individual follicles were dissected out, follicular fluid aspirated, and granulosa and theca cells cultured in vitro. Androstenedione, progesterone and oestradiol content of the follicular fluid and overlying culture medium were measured by radioimmunoassay. There was a significant elevation of both basal and LH-stimulated androstenedione production by theca from both polycystic ovaries (n = 10; P < 0.005) and pelvic congestion cystic ovaries (n = 8; P < 0.05 and < 0.01 respectively) as compared with normal ovaries (n = 5). Granulosa cells from pelvic congestion ovaries (n = 7) had a diminished oestradiol response to FSH as compared with those from normal ovaries (n = 8). Follicular fluid from the majority of follicles in the pelvic congestion cystic ovaries had a high androgen:oestrogen ratio consistent with atresia. For the first time, pelvic congestion ovaries characterized by predominantly atretic follicles scattered throughout the stroma in a normal volume ovary are reported. Follicular atresia was reflected by reduced granulosa cell responsiveness to FSH, theca cell hyperplasia and increased basal and LH-stimulated androgen production. These ovaries are functionally distinct from polycystic ovaries, which do not have a higher proportion of atretic follicles than normal ovaries.
C Gilling-Smith; H Mason; D Willis; S Franks; R W Beard
Related Documents :
21072679 - Bmp-4 suppresses progesterone production by inhibiting histone h3 acetylation of star i...
10628389 - Ovarian cancer and gonadotropins in vitro: new evidence in favor of independence.
9603249 - Differentiation of the corpus luteum of the mink (mustela vison): mitogenic and steroid...
18645009 - S1p differentially regulates migration of human ovarian cancer and human ovarian surfac...
8860319 - Ontogeny of immunoreactive steroidogenic proteins (adrenodoxin and cytochrome p-450(21)...
20149359 - The role of autophagy in follicular development and atresia in rat granulosa cells.
20579099 - Ability of pseudoalteromonas tunicata to colonize natural biofilms and its effect on mi...
23037979 - Photo-killing mechanism of 2-demethoxy-2,3-ethylenediamino hypocrellin b (edahb) to hel...
12437959 - Response of rat osteoblast-like cells to microstructured model surfaces in vitro.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  15     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-03     Completed Date:  2001-01-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2570-6     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Imperial College School of Medicine at St Mary's, London W2 1PG, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Androstenedione / analysis,  biosynthesis
Cells, Cultured
Estradiol / analysis,  biosynthesis
Follicle Stimulating Hormone / pharmacology
Follicular Atresia
Follicular Fluid / chemistry
Granulosa Cells / drug effects,  metabolism
Luteinizing Hormone / pharmacology
Ovary / drug effects,  metabolism*
Progesterone / analysis,  biosynthesis
Steroids / biosynthesis*
Theca Cells / drug effects,  metabolism
Uterus / blood supply*,  ultrasonography
Veins / ultrasonography
Reg. No./Substance:
0/Steroids; 50-28-2/Estradiol; 57-83-0/Progesterone; 63-05-8/Androstenedione; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Clinical management of functional ovarian cysts: a prospective and randomized study.
Next Document:  Refreezing of murine intact and biopsied embryos by rapid-freezing procedure.