| In vitro modifications in the proliferation rate of prolactin cells are accompanied by nuclear morphometric variations. | |
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MedLine Citation:
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PMID: 7756734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to establish the correlation between in vitro proliferation rate and morphometric variations of prolactin immunoreactive cells, a morphometric study was carried out in rat pituitary monolayer cultures by means of the double immunocytochemical staining methods employing mouse monoclonal antiproliferative cell nuclear antigen (PCNA) and rabbit anti-prolactin (PRL) as primary antibodies. PCNA was found to be an adequate marker for proliferation in pituitary monolayer cultures. 48.35 +/- 2.78% of the cells present in the culture were in active cell cycle after 3 days of incubation and a similar proportion, 54.93 +/- 2.83% was found after 7 days. On the 3rd day, PRL immunopositive cells accounted for 15.16 +/- 0.21% of the total cellular content in the dishes and 8.68 +/- 0.12% of the PCNA immunoreactive cells were also PRL immunopositive cells and, 60.95 +/- 2.65% of PRL cells stained for PRL and PCNA. On the 7th day, an increase to 32.18 +/- 0.60% of PRL cells was found; the PCNA and PRL cells accounted for 60.32 +/- 2.34% of the total PRL cells, and 19.88 +/- 1.09% of the PCNA reactive cells stained for PRL. Additionally, the morphometric analysis performed after 3 or 7 days of incubation showed that, while the size of PRL cells remained unmodified, the nuclear area had increased on the 7th day in relation to the 3rd day (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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J Carretero; M Rubio; N Navarro; P Prieto; R J Vázquez; F Sánchez; R Vázquez |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Histology and histopathology Volume: 10 ISSN: 0213-3911 ISO Abbreviation: Histol. Histopathol. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-06-23 Completed Date: 1995-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: SPAIN |
Other Details:
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Languages: eng Pagination: 135-9 Citation Subset: IM |
Affiliation:
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Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division Cell Nucleus / immunology, ultrastructure Immunohistochemistry Male Pituitary Gland, Anterior / cytology*, immunology, metabolism* Prolactin / metabolism* Proliferating Cell Nuclear Antigen / metabolism Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Proliferating Cell Nuclear Antigen; 9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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