Document Detail


In vitro modifications in the proliferation rate of prolactin cells are accompanied by nuclear morphometric variations.
MedLine Citation:
PMID:  7756734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to establish the correlation between in vitro proliferation rate and morphometric variations of prolactin immunoreactive cells, a morphometric study was carried out in rat pituitary monolayer cultures by means of the double immunocytochemical staining methods employing mouse monoclonal antiproliferative cell nuclear antigen (PCNA) and rabbit anti-prolactin (PRL) as primary antibodies. PCNA was found to be an adequate marker for proliferation in pituitary monolayer cultures. 48.35 +/- 2.78% of the cells present in the culture were in active cell cycle after 3 days of incubation and a similar proportion, 54.93 +/- 2.83% was found after 7 days. On the 3rd day, PRL immunopositive cells accounted for 15.16 +/- 0.21% of the total cellular content in the dishes and 8.68 +/- 0.12% of the PCNA immunoreactive cells were also PRL immunopositive cells and, 60.95 +/- 2.65% of PRL cells stained for PRL and PCNA. On the 7th day, an increase to 32.18 +/- 0.60% of PRL cells was found; the PCNA and PRL cells accounted for 60.32 +/- 2.34% of the total PRL cells, and 19.88 +/- 1.09% of the PCNA reactive cells stained for PRL. Additionally, the morphometric analysis performed after 3 or 7 days of incubation showed that, while the size of PRL cells remained unmodified, the nuclear area had increased on the 7th day in relation to the 3rd day (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J Carretero; M Rubio; N Navarro; P Prieto; R J Vázquez; F Sánchez; R Vázquez
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Histology and histopathology     Volume:  10     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-06-23     Completed Date:  1995-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  135-9     Citation Subset:  IM    
Affiliation:
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Cell Nucleus / immunology,  ultrastructure
Immunohistochemistry
Male
Pituitary Gland, Anterior / cytology*,  immunology,  metabolism*
Prolactin / metabolism*
Proliferating Cell Nuclear Antigen / metabolism
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen; 9002-62-4/Prolactin

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