Document Detail

In vitro models for glaucoma research: effects of hydrostatic pressure.
MedLine Citation:
PMID:  21693606     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The response of cells (e.g., optic nerve head [ONH] cells) to mechanical stress is important in glaucoma. Studies have reported the biological effects of hydrostatic pressure on ONH cells cultured on a rigid substrate. An apparatus, designed to independently vary hydrostatic pressure and gas tension (including oxygen tension) in culture medium, was used to evaluate the effects of pressure and tension on cell migration, shape, and α-tubulin architecture in a transformed cell line (DITNC1 rat cortical astrocytes).
METHODS: During the assay period, cells were exposed to one of four experimental configurations: (1) control pressure and control gas tension; (2) high-pressure (7.4 mm Hg) and reduced gas tension; (3) control pressure and reduced gas tension; and (4) high-pressure and control gas tension.
RESULTS: Calculations suggested that the cells in configurations 2 and 3 were hypoxic, as confirmed by direct measurements in configuration 2. No effects of hydrostatic pressure were observed on cell migration or α-tubulin architecture. However, cells cultured under low gas tension (configurations 2 and 3) showed increased migration at 48 and 72 hours (P < 0.05).
CONCLUSIONS: A hydrostatic pressure of 7.4 mm Hg has no effect on DITNC1 astrocytes cultured on rigid coverslips, whereas hypoxia associated with a fluid column creating this pressure does. These results differ from those in a previous report, the results of which may be explained by altered gas tensions in the culture medium. Steps are recommended for control of secondary effects when testing the effect of pressure on cultured cells.
Yuan Lei; Shadi Rajabi; Ryan M Pedrigi; Darryl R Overby; A Thomas Read; C Ross Ethier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-11
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  52     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-12     Completed Date:  2011-10-06     Revised Date:  2013-01-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6329-39     Citation Subset:  IM    
Department of Bioengineering, Imperial College London, London, United Kingdom.
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MeSH Terms
Animals, Newborn
Astrocytes / cytology*,  physiology
Cell Line, Transformed
Cell Movement / physiology*
Cell Shape / physiology*
Culture Media
Diencephalon / cytology
Hydrostatic Pressure*
Models, Biological*
Oxygen / metabolism
Tubulin / metabolism*
Reg. No./Substance:
0/Culture Media; 0/Tubulin; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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