| In vitro models for glaucoma research: Effects of hydrostatic pressure. | |
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MedLine Citation:
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PMID: 21693606 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: The response of cells to mechanical stress is important, e.g. for optic nerve head (ONH) cells in glaucoma. Studies have reported biological effects of hydrostatic pressure on ONH cellscultured on a rigid substrate. Here we designed apparatus to independently varyhydrostatic pressure and culture medium gas tension (including oxygen tension), and used this apparatus to evaluate hydrostatic pressure and gas tension effects on cell migration, shape and alpha-tubulin architecture in a transformed cell line (DI TNC1 rat cortical astrocytes). Methods: During the assay period, cells were exposed to one of four experimental configurations: 1) control pressure, control gas tension; 2) high pressure (7.4 mmHg), reduced gas tension; 3) control pressure, reduced gas tension; 4) high pressure, control gas tension. Results: Calculations suggested that cells in Configurations 2 and 3 were hypoxic, as confirmed by direct measurements in Configuration 2.No effects of hydrostatic pressure were observed on cell migration or alpha-tubulin architecture. However, cells cultured under low gas tension (Configurations 2 and 3) showed increased migration at 48 and 72 hrs (p < 0.05). Conclusions: A hydrostatic pressure of 7.4 mmHg has no effect on DI TNC1 astrocytes cultured on rigid coverslips, while hypoxia associated with a fluid column creating this pressure does. These results differ from a previous report (Salvador-Silva et al., Glia, 2004), the results of which may be explained by altered gas tensions in the culture medium. We recommend steps for control of secondary effects when testing the effect of pressure on cultured cells. |
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Authors:
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Yuan Lei; Shadi Rajabi; Ryan M Pedrigi; Darryl R Overby; A Thomas Read; C Ross Ethier |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-21 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: - ISSN: 1552-5783 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Bioengineering, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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