Document Detail

In vitro model of neurotoxicity of Abeta 1-42 and neuroprotection by a pentapeptide: irreversible events during the first hour.
MedLine Citation:
PMID:  15571986     Owner:  NLM     Status:  MEDLINE    
The cell biology of Alzheimer's disease (AD) is characterized mainly by the neurodegeneration caused by the beta-amyloid (Abeta) peptides and by the formation of neurofibrillary tangles. The initial events of neurodegeneration in the brain tissue include synaptic dysfunction and axonopathy. Abeta-induced axonopathy and neurite degeneration were studied in vitro on differentiated human-derived neurotypic SH-SY5Y cells. Different methods were used to investigate the mechanism of action of aggregated Abeta on neuroblastoma cells. Abeta 1-42 aggregated for 1 h induced irreversible changes in the neurite morphology. Change of tau hyperphosphorylation and cell viability (cytoplasmic redox state and active membrane uptake) was irreversible during the first hour after the addition of Abeta 1-42 to the cells. These rapid events indicate that Abeta might induce neurodegeneration even at an early stage of Abeta-cell contact. A novel pentapeptide LPYFD-amide, an analog of Soto's LPFFD, significantly decreased neurite degeneration, tau aggregation, and cell viability reduction induced by Abeta 1-42.
Zsolt Datki; Rita Papp; Dénes Zádori; Katalin Soós; Lívia Fülöp; Anna Juhász; Gábor Laskay; Csaba Hetényi; Erzsébet Mihalik; Márta Zarándi; Botond Penke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurobiology of disease     Volume:  17     ISSN:  0969-9961     ISO Abbreviation:  Neurobiol. Dis.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-01     Completed Date:  2005-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9500169     Medline TA:  Neurobiol Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  507-15     Citation Subset:  IM    
Department of Medical Chemistry, University of Szeged, Szeged, Hungary.
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MeSH Terms
Alzheimer Disease / pathology
Amyloid beta-Protein / chemistry,  toxicity*
Cell Line, Tumor
Microscopy, Electron, Scanning
Nerve Tissue Proteins / drug effects,  metabolism
Neurofibrils / drug effects,  ultrastructure
Neurotoxins / toxicity
Peptide Fragments / therapeutic use*
Time Factors
Reg. No./Substance:
0/Amyloid beta-Protein; 0/Nerve Tissue Proteins; 0/Neurotoxins; 0/Peptide Fragments

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