| In vitro model of neurotoxicity of Abeta 1-42 and neuroprotection by a pentapeptide: irreversible events during the first hour. | |
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MedLine Citation:
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PMID: 15571986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cell biology of Alzheimer's disease (AD) is characterized mainly by the neurodegeneration caused by the beta-amyloid (Abeta) peptides and by the formation of neurofibrillary tangles. The initial events of neurodegeneration in the brain tissue include synaptic dysfunction and axonopathy. Abeta-induced axonopathy and neurite degeneration were studied in vitro on differentiated human-derived neurotypic SH-SY5Y cells. Different methods were used to investigate the mechanism of action of aggregated Abeta on neuroblastoma cells. Abeta 1-42 aggregated for 1 h induced irreversible changes in the neurite morphology. Change of tau hyperphosphorylation and cell viability (cytoplasmic redox state and active membrane uptake) was irreversible during the first hour after the addition of Abeta 1-42 to the cells. These rapid events indicate that Abeta might induce neurodegeneration even at an early stage of Abeta-cell contact. A novel pentapeptide LPYFD-amide, an analog of Soto's LPFFD, significantly decreased neurite degeneration, tau aggregation, and cell viability reduction induced by Abeta 1-42. |
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Authors:
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Zsolt Datki; Rita Papp; Dénes Zádori; Katalin Soós; Lívia Fülöp; Anna Juhász; Gábor Laskay; Csaba Hetényi; Erzsébet Mihalik; Márta Zarándi; Botond Penke |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurobiology of disease Volume: 17 ISSN: 0969-9961 ISO Abbreviation: Neurobiol. Dis. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-12-01 Completed Date: 2005-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9500169 Medline TA: Neurobiol Dis Country: United States |
Other Details:
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Languages: eng Pagination: 507-15 Citation Subset: IM |
Affiliation:
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Department of Medical Chemistry, University of Szeged, Szeged, Hungary. datkiz@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alzheimer Disease
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pathology Amyloid beta-Protein / chemistry, toxicity* Cell Line, Tumor Humans Microscopy, Electron, Scanning Nerve Tissue Proteins / drug effects, metabolism Neurofibrils / drug effects, ultrastructure Neurotoxins / toxicity Peptide Fragments / therapeutic use* Phosphorylation Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amyloid beta-Protein; 0/Nerve Tissue Proteins; 0/Neurotoxins; 0/Peptide Fragments |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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