| In vitro model for the assessment of luminal factors on rectal mucosa. | |
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MedLine Citation:
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PMID: 6884817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An organ culture method for the maintenance of rectal biopsies over a period of 24 hours is described. Good preservation of histological architecture and continued crypt cell proliferation were shown over the culture period. The colonic enzyme alkaline phosphatase was found to rise over the period of culture. This rise was dependent upon continued protein synthesis by the cell. Changes in alkaline phosphatase activity during culture in biopsies from patients with ulcerative colitis and Crohn's disease are reported. This organ culture system and the measurement of alkaline phosphatase activity during culture provides a new approach to the assessment of luminal antigens as possible effectors of colonic epithelial cell damage. |
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Authors:
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A Allan; D P Jewell |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gut Volume: 24 ISSN: 0017-5749 ISO Abbreviation: Gut Publication Date: 1983 Sep |
Date Detail:
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Created Date: 1983-10-21 Completed Date: 1983-10-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985108R Medline TA: Gut Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 812-7 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Colitis, Ulcerative / enzymology, pathology* Crohn Disease / enzymology, pathology* Humans Levamisole / pharmacology Organ Culture Techniques Puromycin / pharmacology Rectum / drug effects, enzymology, pathology* |
| Chemical | |
Reg. No./Substance:
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14769-73-4/Levamisole; 53-79-2/Puromycin; EC 3.1.3.1/Alkaline Phosphatase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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