| In-vitro methods to decrease MHC class II-positive cells in retinal pigment epithelium cell grafts. | |
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MedLine Citation:
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PMID: 9785604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rejection of RPE transplants may be caused by expression of MHC class II molecules on the graft. We wanted to determine if passage level, hyperoxia, low temperature, or magnetic separation had an influence on this expression or on several other physiological parameters of bovine RPE cells. For this, bovine RPE cells were cultured under normal conditions for several passages (1-5) or were incubated with a high level of oxygen (75%) for 24 h or at low temperature (24 degrees C) for seven days. Magnetic beads coated with monoclonal antibody against MHC class II antigens were used to separate the positive cells from the total cell population. The number of MHC class II-positive RPE cells was not affected by increased passage, oxygen concentration, or low temperature. Using magnetic beads, approximately 7.5% of the cells were separated from the total RPE population as MHC class II-positive cells. Hyperoxia initially increased the number of RPE cells to 178% that of the untreated controls, but the subsequent MTT test showed a decrease in proliferation to 95% of control levels. Similarly, low temperature increased cell number of 110% that of controls, but prolonged proliferation decreased to 76% of the controls. None of the experimental conditions had a significant influence on the viability of the cells. Culture conditions can be modified to increase the yield of RPE cells, and MHC class II-positive RPE cells can be detected and selectively separated from the total cell population, both of which may prove to be useful for RPE cell transplantation. |
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Authors:
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V Enzmann; M Stadler; P Wiedemann; L Kohen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Ocular immunology and inflammation Volume: 6 ISSN: 0927-3948 ISO Abbreviation: Ocul. Immunol. Inflamm. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-12-03 Completed Date: 1998-12-03 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9312169 Medline TA: Ocul Immunol Inflamm Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 145-53 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Faculty of Medicine, University of Leipzig, Germany. enzv@server3.medizin.uni-leipzig.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell Count Cell Division Cell Separation Cell Transplantation Cells, Cultured Cold Temperature Fluorescent Antibody Technique, Indirect Histocompatibility Antigens Class II / metabolism* Oxygen / metabolism Pigment Epithelium of Eye / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens Class II; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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