Document Detail

In vitro measurement of post-natal changes in proliferating satellite cell frequency during rat muscle growth.
MedLine Citation:
PMID:  20438507     Owner:  NLM     Status:  MEDLINE    
Satellite cells, resident myogenic stem cells found in postnatal skeletal muscle, are most abundant during early postnatal development and sharply decline in frequency thereafter to adult levels in mice and rats. Therefore, postnatal changes in satellite cell mitotic activities are important aspects for further understanding a muscle growth strategy. In large meat-production animals, however, the traditional in vivo proliferation assay may be less realistic because it requires intra-peritoneal (ip) injection of huge dosage of mutagenic nucleosides, (3)H-labeled thymidine or bromodeoxyuridine (BrdU), at each age-time of sacrifice. We report in the present pilot study using rats that in vivo proliferation activity of satellite cells can be evaluated by an in vitro BrdU-incorporation assay in early cultures. Briefly, satellite cells were prepared from upper hind-limb and back muscles and maintained for 24 h with imposing by BrdU addition for the last 2 h, followed by the regular immunocytochemistry for determining BrdU-incorporated cell percentage. This in vitro assay demonstrated a rapid decrease in proliferating satellite cell frequency to the adult level during about 3-month period after birth, and yielded a high correlation to the measurements by the in vivo BrdU ip-injection method during the postnatal period examined from day-2 to month-11. The in vitro proliferation assay may be further adaptable for large domestic animals by the combination with a muscle biopsy technique that enables age-interval sampling from the same growing animals.
Takahiro Suzuki; Hideyuki Takaishi; Tomowa Sakata; Mai-Khoi Q Do; Minako Hara; Akiko Sato; Wataru Mizunoya; Takanori Nishimura; Akihito Hattori; Yoshihide Ikeuchi; Ryuichi Tatsumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Animal science journal = Nihon chikusan Gakkaih?     Volume:  81     ISSN:  1740-0929     ISO Abbreviation:  Anim. Sci. J.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-04     Completed Date:  2010-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956805     Medline TA:  Anim Sci J     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  245-51     Citation Subset:  IM    
Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kyushu University, Higashi, Fukuoka and.
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MeSH Terms
Bromodeoxyuridine / metabolism
Cell Proliferation
Cytological Techniques
Muscle, Skeletal / cytology*,  growth & development*
Pilot Projects
Rats, Sprague-Dawley
Satellite Cells, Skeletal Muscle / cytology*
Reg. No./Substance:

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