| In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary. | |
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MedLine Citation:
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PMID: 20737203 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: create a 3-Dimensional artificial human ovary to mature human oocytes. METHODS: theca and granulosa cells were isolated from antral follicles of reproductive-aged women, seeded into micro-molded gels and self-assembled into complex 3D microtissues. Immunohistochemistry and live-dead staining confirmed theca cell identity and cellular viability at one week respectively. Placement of granulosa cell spheroids or cumulus-oocyte complexes into theca cell honeycomb openings resulted in creation of an artificial human ovary. Oocytes from this construct were assessed for polar body extrusion. RESULTS: theca and granulosa cells self-assembled into complex microtissues, remaining viable for one week. At 72 h after artificial human ovary construction, theca cells completely surrounded the granulosa spheroids or COCs without stromal invasion or disruption. Polar body extrusion occurred in one of three COCs assessed. CONCLUSIONS: an artifical human ovary can be created with self-assembled human theca and granulosa cell microtissues, and used for IVM and future oocyte toxicology studies. |
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Authors:
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Stephan P Krotz; Jared C Robins; Toni-Marie Ferruccio; Richard Moore; Margaret M Steinhoff; Jeffrey R Morgan; Sandra Carson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-25 |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: 27 ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-07 Completed Date: 2011-03-24 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: Netherlands |
Other Details:
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Languages: eng Pagination: 743-50 Citation Subset: IM |
Affiliation:
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Division of Reproductive Endocrinology & Infertility, Department of Obstetrics & Gynecology, Women & Infants' Hospital, Alpert Medical School, Brown University, Providence, RI 02905, USA. StephanKrotzMD@gmail.com |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Artificial Organs* Female Fertilization in Vitro Granulosa Cells / cytology Humans Middle Aged Oocytes / cytology* Ovarian Follicle / cytology Ovariectomy Ovary / cytology* Spheroids, Cellular / cytology Theca Cells / metabolism Tissue Engineering* |
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