Document Detail


In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary.
MedLine Citation:
PMID:  20737203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: create a 3-Dimensional artificial human ovary to mature human oocytes.
METHODS: theca and granulosa cells were isolated from antral follicles of reproductive-aged women, seeded into micro-molded gels and self-assembled into complex 3D microtissues. Immunohistochemistry and live-dead staining confirmed theca cell identity and cellular viability at one week respectively. Placement of granulosa cell spheroids or cumulus-oocyte complexes into theca cell honeycomb openings resulted in creation of an artificial human ovary. Oocytes from this construct were assessed for polar body extrusion.
RESULTS: theca and granulosa cells self-assembled into complex microtissues, remaining viable for one week. At 72 h after artificial human ovary construction, theca cells completely surrounded the granulosa spheroids or COCs without stromal invasion or disruption. Polar body extrusion occurred in one of three COCs assessed.
CONCLUSIONS: an artifical human ovary can be created with self-assembled human theca and granulosa cell microtissues, and used for IVM and future oocyte toxicology studies.
Authors:
Stephan P Krotz; Jared C Robins; Toni-Marie Ferruccio; Richard Moore; Margaret M Steinhoff; Jeffrey R Morgan; Sandra Carson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-25
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  27     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-07     Completed Date:  2011-03-24     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  743-50     Citation Subset:  IM    
Affiliation:
Division of Reproductive Endocrinology & Infertility, Department of Obstetrics & Gynecology, Women & Infants' Hospital, Alpert Medical School, Brown University, Providence, RI 02905, USA. StephanKrotzMD@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Artificial Organs*
Female
Fertilization in Vitro
Granulosa Cells / cytology
Humans
Middle Aged
Oocytes / cytology*
Ovarian Follicle / cytology
Ovariectomy
Ovary / cytology*
Spheroids, Cellular / cytology
Theca Cells / metabolism
Tissue Engineering*
Comments/Corrections

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