Document Detail


In vitro maturation and intracytoplasmic sperm injection of oocytes collected from hormonally stimulated common wombats, Vombatus ursinus.
MedLine Citation:
PMID:  16644150     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Porcine FSH/LH stimulation successfully induced development of multiple large (>or=4mm) antral follicles in 10 of 11 common wombats. A mean of 5.5 metaphase II (MII) oocytes were aspirated from wombats that were stimulated during the follicular phase of the oestrous cycle (n=3) or after pouch young removal (n=3). Three subadults (n=3) and two anoestrus adults did not produce MII oocytes despite pFSH/pLH administration. In vitro maturation of immature oocytes at the time of aspiration doubled the number of MII oocytes that could be collected from pFSH/pLH stimulated wombats. Immature oocytes with cumulus attached, matured more readily to the MII stage than immature oocytes without cumulus. Following intracytoplasmic sperm injection (ICSI), approximately 5% of the oocytes that were MII at the time of collection cleaved. Approximately 5% of those that were matured by in vitro maturation (IVM) formed two polar bodies following ICSI, although they not cleave. Parthenogenesis cannot be excluded. This demonstrates that assisted reproductive technologies may be applicable to the common wombat.
Authors:
M West; O Lacham-Kaplan; M Cleary; D Galloway; J Shaw; A O Trounson; M C J Paris
Publication Detail:
Type:  Journal Article     Date:  2006-04-27
Journal Detail:
Title:  Animal reproduction science     Volume:  98     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-02-19     Completed Date:  2007-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  311-21     Citation Subset:  IM    
Affiliation:
Monash Institute of Reproduction and Development 27-31 Wright St, Clayton, Vic. 3168, Australia. mwest@zoo.org.au
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Follicle Stimulating Hormone / pharmacology
Luteinizing Hormone / pharmacology
Male
Marsupialia / physiology*
Oocytes / cytology*,  drug effects
Ovary / anatomy & histology,  drug effects
Sperm Injections, Intracytoplasmic / veterinary*
Swine
Chemical
Reg. No./Substance:
9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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