Document Detail


In vitro maturation and fertilization of oocytes recovered from free-ranging Burchell's zebra (Equus burchelli) and Hartmann's zebra (Equus zebra hartmannae).
MedLine Citation:
PMID:  9365936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A noninvasive repeatable method to harvest oocytes for in vitro fertilization (IVF) could potentially be used to assist reproduction in endangered equid species. The objectives of this study were to evaluate a specific transvaginal ultrasound-guided oocyte recovery procedure for use in zebra mares and the general applicability of IVF procedures in zebra. Ovaries were collected from Burchell's zebra (Equus burchelli) and Hartmann's zebra (Equus zebra hartmannae) mares at routine culling for Expt. I. Of the 144 oocytes recovered from these ovaries, 70% were of excellent quality. No significant difference in oocyte quality was found between the two zebra species. Zona drilling was performed on in vitro-matured oocytes prior to IVF. Epididymal sperm from culled Burchell's zebra stallions were used for IVF. The sperm either were exposed to calcium ionophore or were not treated and served as a control. In vitro fertilized oocytes were then co-cultured with zebra granulosa cells (ZGC) or with bovine oviduct cells (BOC) for up to 8 days. Overall, a 38% cleavage rate was obtained with 16% of sperm-exposed oocytes developing to the morula or blastocyst stage. All of the embryos that developed to at least the morula stage were cultured on BOC; whereas, none of those cultured on ZGC reached the morula stage during the same interval. Cleavage rates of oocytes inseminated with ionophore-treated or with control sperm were not significantly different, suggesting that ionophore treatment of epididymal sperm for IVF in these zebra species may be redundant. In Expt. II, 10 transvaginal ultrasound-guided oocyte aspiration procedures on five captive Burchell's zebra mares recovered a total of 33 oocytes (6.6 oocytes/female) of which 94% were considered viable. This approach may be an attractive means of producing gametes for assisted reproduction in endangered species. Furthermore, results from this study indicate that IVF may become a means of producing offspring from zebra and other equid species in the future.
Authors:
M Meintjes; C Bezuidenhout; P Bartels; D S Visser; J Meintjes; N M Loskutoff; F L Fourie; D M Barry; R A Godke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians     Volume:  28     ISSN:  1042-7260     ISO Abbreviation:  J. Zoo Wildl. Med.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-02-05     Completed Date:  1998-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8915208     Medline TA:  J Zoo Wildl Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  251-9     Citation Subset:  IM    
Affiliation:
Department of Physiology, Pharmacology and Toxicology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Coculture Techniques / methods,  veterinary
Embryonic and Fetal Development / physiology
Equidae / physiology*
Fallopian Tubes / cytology,  physiology
Female
Fertilization in Vitro / methods,  veterinary*
Male
Oocyte Donation / methods,  veterinary*
Oocytes / cytology,  physiology*,  ultrasonography
Pregnancy
Pregnancy, Animal / physiology*
South Africa
Sperm-Ovum Interactions / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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