Document Detail

In vitro magnetic resonance microimaging of experimental osteoarthritis in the rat knee joint.
MedLine Citation:
PMID:  9002024     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To visualize articular changes during iodoacetate induced osteoarthritis (OA) of the rat knee using high resolution magnetic resonance imaging (MRI). METHODS: All images were acquired on a 8.5T microimager on ex vivo rat knees. After optimization studies, 3D spin echo sequences were used with TR 1200 ms and TE 15 ms; 40 sagittal images were obtained with a resolution of 400 x 60 x 60 microns. OA lesions were achieved by injecting 3 mg iodoacetate in the right knee joint (Day 0). Progression of OA changes was studied at Days 5, 10, 15, 20, 30, 90 and corresponding radiographs and histological sections were obtained. RESULTS: Spin echo images of the normal rat knee clearly visualized cortical bone, calcified menisci, patella, and cruciate ligaments as dark regions. Cartilage and muscles appeared as intermediate signal. In some cases a trilaminar appearance of cartilage and growth plate was depicted. This appearance persisted at Day 5, although cartilage presented a marked loss of proteoglycans. After Day 10 the menisci appeared irregular and inhomogeneous. After Day 15, MRI showed important alterations of articular cartilage, predominant on weight bearing areas of femoral condyles. Osteophytic remodeling was also seen around the patella. From Day 20 to Day 90 anatomical changes progressively affected epiphyseal bone, leading to subchondral cysts and loss of its regular trabecular structure. CONCLUSION: High field MRI provides a sensitive method for investigating ex vivo focal erosions of cartilage and established osteochondral remodeling in experimental OA in the rat. In this model, microimaging provides more information about early modifications of cartilage and soft tissue than radiographic exploration, in good correlation with histological data.
D Loeuille; P Gonord; C Guingamp; P Gillet; A Blum; M Sauzade; P Netter
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of rheumatology     Volume:  24     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-26     Completed Date:  1997-03-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  133-9     Citation Subset:  IM    
Laboratoire de Pharmacologie and Clinique Rhumatologique, Faculté de Médecine, URA CNRS 1288 Vandoeuvre les Nancy, France.
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MeSH Terms
Injections, Intra-Articular
Iodoacetates / administration & dosage
Iodoacetic Acid
Knee Joint / pathology*,  radiography
Magnetic Resonance Imaging*
Osteoarthritis / chemically induced,  pathology*,  radiography
Rats, Wistar
Reg. No./Substance:
0/Iodoacetates; 64-69-7/Iodoacetic Acid

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