Document Detail


In vitro kinetics of soybean lipoxygenase with combinatorial fatty substrates and its functional significance in off flavour development.
MedLine Citation:
PMID:  24176359     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Lipoxygenase (Lox) mediated oxidation of polyunsaturated fatty acids (PUFA) in mature soya seeds results in objectionable flavour. In the present study, Lox isozymes were purified to near homogeneity (107-fold). Lox-2 and 3 displayed remarkable kinetic preference (1.7 and 1.5-fold, respectively) for low PUFA ratios (LA/LeA) (PRs) among the selected PUFA combinations. Lox-1 displayed no specific preference. Pure Lox-1 displayed unbiased response towards substrates with marginal preference (1.2-fold) for linoleic acid at its optimum pH. Volatile compounds profiling showed a direct relationship between PRs and hexanal to trans-2-hexenal (1.47, 2.24 and 18.90 for 2, 7 and 15 PRs, respectively) ratio. The off-flavour determining parameters like TBA value, carbonyl value and lipid hydroperoxides (LHPODs) exhibited significant negative correlation (0.76, 0.74, 0.72; p<0.0001) in selected soya genotypes displaying varied PRs and significant positive correlation (0.89, 0.81. 0.89; p<0.0001) with ratio of PI (polyene index) to PRs - suggesting the plausible significance of PUFA ratios in biological lipid peroxidation.
Authors:
Somnath Mandal; Anil Dahuja; Abhijit Kar; I M Santha
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Publication Detail:
Type:  Journal Article     Date:  2013-09-17
Journal Detail:
Title:  Food chemistry     Volume:  146     ISSN:  0308-8146     ISO Abbreviation:  Food Chem     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2013-11-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702639     Medline TA:  Food Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  394-403     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Ltd. All rights reserved.
Affiliation:
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: somnathmandal@jncasr.ac.in.
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