| In vitro investigation of maleated poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) for its biocompatibility to mouse fibroblast L929 and human microvascular endothelial cells. | |
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MedLine Citation:
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PMID: 18306313 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), a polyester with strong mechanical properties and biocompatibility, is a member of microbial polyhydroxyalkanoates (PHA) family. Maleic anhydride was used to graft PHBHHx to form maleated PHBHHx (Ma-PHBHHx). Ma-PHBHHx with a graft degree of 0.59% was found to be more thermo-stable in comparison with PHBHHx. In vitro study demonstrated that the biocompatibility to mouse fibroblast L929 and human microvascular endothelial cells (HMEC) was improved in different degrees on Ma-PHBHHx films and scaffolds. Compared with their growth on PHBHHx, L929 and HMEC grown on Ma-PHBHHx films and scaffolds showed approximately 120% and 260% more in proliferation rates, respectively. Morphology study suggested that fine whorl-like surface structures with porosities on Ma-PHBHHx films attributed to MA grafting would be better for cell attachment and proliferation. Ma-PHBHHx scaffolds prepared by thermally induced phase separation (TIPS) with increased porosity, hydrophilicity, surface energy, and charges also were more favorable for cell growth. In addition, Ma-PHBHHx showed an accelerated degradation incubation in SBF at 37 degrees C, losing 21.4% of its original weight after 21 weeks while PHBHHx just lost 7.3%. Based on the improved biocompatibility, reasonable mechanical properties as well as accelerated biodegradation, Ma-PHBHHx has shown advantages over PHBHHx as a biomaterial for biomedical applications. |
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Authors:
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Xiao-Tao Li; Jie Sun; Song Chen; Guo-Qiang Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 87 ISSN: 1552-4965 ISO Abbreviation: - Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-03 Completed Date: 2009-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 832-42 Citation Subset: IM |
Copyright Information:
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(c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008. |
Affiliation:
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Multidisciplinary Research Center, Shantou University, Shantou 515063, Guangdong, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxybutyric Acid
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chemistry* Animals Biocompatible Materials / chemistry* Cell Adhesion Cell Line Cell Proliferation Hexanoic Acids / chemistry* Humans Maleic Anhydrides / chemistry Mice Microscopy, Confocal Porosity Surface Properties Tissue Engineering Tissue Scaffolds* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Hexanoic Acids; 0/Maleic Anhydrides; 0/poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); 300-85-6/3-Hydroxybutyric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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