Document Detail


In vitro invasiveness of CTL clones and in vivo dissemination of CTL hybridomas.
MedLine Citation:
PMID:  8482918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Activated spleen T cells are invasive in hepatocyte and fibroblast cultures, and this property is dominantly expressed in T cell hybridomas. The invasive potential of the hybrids correlates with their capacity to disseminate in vivo. We have used this model to study the invasive and migratory properties of cytotoxic T lymphocytes (CTLs). Two murine CTL clones were highly invasive, independent of their state of activation. CTL hybridomas, derived from one of the clones, were similarly invasive. In vivo, CTL hybridoma cells disseminated to extravascular sites in the liver, kidneys, lungs, ovaria, tubae, uterus, and lymphoid, mesenchymal, and fat tissues. Within 7 to 14 days, 10(6) cells were lethal in 100% of mice. The adhesion molecules CD2, CD8, CD54, L-selectin, and CD49d (VLA-4 and LPAM-1 alpha-chain) were not expressed by all CTL hybridomas and therefore not indispensable for invasion in vitro and dissemination in vivo. In contrast, LFA-1 (CD11a/CD18), CD44, and VLA-6 (CD49f/CD29) were expressed on all hybrids. LFA-1 antibodies inhibited CTL hybridoma invasion in vitro, but antibodies inhibiting CD44-hyaluronate and VLA-6-laminin interaction had no effect. These results suggest that migration of cytotoxic T cells into noninflamed tissues is independent of their activation state and does not require L-selectin, LPAM-1, CD2, and VLA-4.
Authors:
G La Rivière; J W Gebbinck; C A Schipper; W J Mooi; E Roos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  53     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-03     Completed Date:  1993-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
Affiliation:
Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Antigens, Differentiation, T-Lymphocyte / analysis*
Cell Adhesion Molecules / analysis
Cell Movement
Cells, Cultured
Clone Cells
DNA / analysis
Histocompatibility Antigens Class II / analysis
Hybridomas / pathology,  physiology
Liver / immunology*
Lymphocyte Activation
Mice
Neoplasm Invasiveness
Neoplasm Metastasis
Organ Specificity
Spleen / immunology
T-Lymphocytes, Cytotoxic / immunology,  physiology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation, T-Lymphocyte; 0/Cell Adhesion Molecules; 0/Histocompatibility Antigens Class II; 9007-49-2/DNA

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