| In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor. | |
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MedLine Citation:
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PMID: 21472452 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction of G0/G1 and G2/M -phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent for the treatment of canine MCT. |
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Authors:
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Marcia Kazumi Nagamine; Daniel S Sanches; Katia C Pinello; Luciana Neves Torres; Gregory Mennecier; Andréia O Latorre; Heidge Fukumasu; Maria Lucia Zaidan Dagli |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-7 |
Journal Detail:
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Title: Veterinary research communications Volume: - ISSN: 1573-7446 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100520 Medline TA: Vet Res Commun Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Laboratory of Experimental Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-900, São Paulo, SP, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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