Document Detail


In vitro inhibitory effect on porphyrinogen carboxylyase of liver extracts from TCDD treated mice.
MedLine Citation:
PMID:  6695412     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Marked inhibition of porphyrinogen carboxylyase was produced in vitro by cytosol fractions, deproteinized and free of porphyrins, obtained from livers of mice made porphyric by 9 weeks i.p. treatment with 25 micrograms/kg/week of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Inhibition was proportional to the amount of the fraction added, was increased by preincubation in the absence of the substrate and, once established, could not be reversed by dialysis. TCDD itself, added to the control enzyme in the incubation mixture, did not affect enzyme activity up to a concentration of 77 nM, which is 10 times higher than the liver TCDD concentration found after in vivo TCDD treatment.
Authors:
L Cantoni; D dal Fiume; M Rizzardini; R Ruggieri
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicology letters     Volume:  20     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-03-15     Completed Date:  1984-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  211-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carboxy-Lyases / antagonists & inhibitors*
Cytosol / drug effects,  enzymology
Decarboxylation
Dioxins / pharmacology*
Liver / drug effects,  enzymology*
Male
Mice
Mice, Inbred C57BL
Tetrachlorodibenzodioxin / pharmacology*
Uroporphyrinogens / metabolism
Chemical
Reg. No./Substance:
0/Dioxins; 0/Uroporphyrinogens; 1746-01-6/Tetrachlorodibenzodioxin; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.-/porphyrinogen carboxy-lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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