Document Detail


In vitro inhibitory effect of flavonoids on growth, infection and vacuolation of Helicobacter pylori.
MedLine Citation:
PMID:  15770537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Flavonoids, which are main constituents of herbal medicines, have been reported to inhibit the growth of Helicobacter pylori (HP). Therefore, to evaluate the anti-HP activity of some flavonoids (flavanols, flavones, flavonols and isoflavonoids), their effects on the growth and vacuolation of HP as well as the infective properties of HP against HeLa cells were investigated. Catechins, quercetin and naringenin weakly inhibited the growth of HP, but all tested compounds did not inhibit HP infection into KATO III cells and HP urease activity. Quercetin and naringenin inhibited HP VacA vacuolation in HeLa cells with IC (50) values of 0.046 and 0.36 mM, respectively. Quercetin also inhibited procaspase-3 activation to caspase-3 in HeLa cells induced by HP VacA toxin, which may induce cell death via the proteolytic activation of a cascade of caspases. However, quercetin did not affect Bax and Bcl-2 protein levels. Based on these findings, quercetin may improve gastric cell death by inhibiting apoptotic signaling by HP VacA toxin. Abbreviations. HP: Helicobacter pyloriBSA:bovine serum albumin ESL:enhanced chemiluminescence MIC:minimum inhibitory concentration MTT:methylthiazolyldiphenyl-tetrazolium bromide PBS:phosphate-buffered saline VacA:Vacuolating cytotoxin.
Authors:
Ji-Eun Shin; Jong-Mi Kim; Eun-Ah Bae; Yang-Jin Hyun; Dong-Hyun Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  71     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-16     Completed Date:  2005-04-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  197-201     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Kyung Hee University, 1 Hoegi, Dongdaemun-ku, Seoul 130-701, Korea.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / administration & dosage,  pharmacology*,  therapeutic use
Bacterial Proteins / metabolism
Bacterial Toxins / metabolism
Flavonoids / administration & dosage,  pharmacology*,  therapeutic use
Hela Cells / microbiology
Helicobacter pylori / drug effects*,  growth & development,  pathogenicity
Humans
Inhibitory Concentration 50
Phytotherapy*
Plant Extracts / administration & dosage,  pharmacology,  therapeutic use
Plants, Medicinal*
Vacuoles / metabolism
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Bacterial Toxins; 0/Flavonoids; 0/Plant Extracts; 0/VacA protein, Helicobacter pylori

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