Document Detail

In vitro inhibition of low density lipoprotein carbamylation by vitamins, as an ameliorating atherosclerotic risk in uremic patients.
MedLine Citation:
PMID:  20156036     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Previous studies have shown that the increase of carbamylated LDL (cLDL), a product of nonenzymatic modification of LDL in human serum by urea-derived cyanate, may cause cardiovascular complications in patients with chronic renal insufficiency. This study examined the inhibitory effect of ascorbic acid, alpha-tocopherol and lycopene on LDL carbamylation in an in vitro model system. METHODS: After isolation of LDL from plasma using an ultracentrifuge technique, cyanate was added to it and then LDL carbamylation was measured in both the absence and presence of ascorbic acid, alpha-tocopherol and/or lycopene by the colorimetric method at 530 nm. RESULTS: The findings indicated that these vitamins inhibit LDL carbamylation and the most effective vitamin of the three is lycopene. Moreover, the effect of lycopene on this process increased in the presence of ascorbic acid and alpha-tocopherol. CONCLUSION: This study indicated that ascorbic acid, alpha-tocopherol and lycopene with antioxidant activity can probably inhibit LDL carbamylation and therefore may have a role in ameliorating atherosclerotic risk of patients with kidney failure. However in vitro and in vivo investigations are required to confirm the exact effects of these vitamins on patients suffering from uremic disorders.
Mohammad Ali Ghaffari; Mehrnoosh Shanaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  70     ISSN:  1502-7686     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-19     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  England    
Other Details:
Languages:  eng     Pagination:  122-7     Citation Subset:  IM    
Department of Clinical Biochemistry, Cellular and Molecular Research Center & Physiology Research Center, Ahwaz Jundishapour University of Medical Sciences, Ahwaz, Iran.
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MeSH Terms
Ascorbic Acid / pharmacology
Atherosclerosis / etiology,  prevention & control*
Carotenoids / pharmacology
Citrulline / analogs & derivatives,  analysis
Cyanates / metabolism*
Electrophoresis, Agar Gel
Lipoproteins, LDL / chemistry,  metabolism*
Protein Processing, Post-Translational / drug effects*
Uremia / complications,  metabolism*
Vitamins / pharmacology*
Young Adult
alpha-Tocopherol / pharmacology
Reg. No./Substance:
0/Cyanates; 0/Lipoproteins, LDL; 0/Vitamins; 0/carbamyl-LDL; 1190-49-4/homocitrulline; 36-88-4/Carotenoids; 372-75-8/Citrulline; 50-81-7/Ascorbic Acid; 502-65-8/lycopene; 59-02-9/alpha-Tocopherol; 590-28-3/potassium cyanate

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