| In vitro and in vivo transformation in rat tracheal epithelial cells exposed to diesel emission particles and related compounds. | |
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MedLine Citation:
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PMID: 9600696 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rat tracheal epithelial (RTE) cell transformation assay was performed to determine the transforming activity of diesel emission particles (DEPs) and two related compounds, 1-nitropyrene (1-NP) and dibenzo(a,i)pyrene (DBP). RTE cells were treated with these agents in vitro and in vivo. Transformed cells from foci induced by these agents were passaged over 20 times to establish immortal cell lines. Results show that (1) DEPs- and 1-NP-induced cell transformation only with the in vivo exposure (30-75 mg/kg bw DEPs and 15-60 mg/kg bw 1-NP); (2) positive dose-related responses to DBP were found with both in vitro (0.05-0.50 microg/ml) and in vivo (7.5-30 mg/kg bw) exposures; (3) the fraction of transformed foci becoming cell lines was in the order of 1-NP(25/48) > DBP(8/28) > DEPs(0/30). These results indicate that (1) DEPs, 1-NP and DBP are capable of transforming rat tracheal epithelial cells, however, the transforming activity of DEPs and -NP may be dependent on metabolic activation, and (2) transformed cells induced by DEPs have a very low probability, if any, of becoming cell lines. |
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Authors:
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M X Ensell; W Z Whong; Z C Heng; J Nath; T Ong |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutation research Volume: 412 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-06-04 Completed Date: 1998-06-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 283-91 Citation Subset: IM |
Affiliation:
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West Virginia University, Morgantown, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzopyrenes / toxicity Cell Line, Transformed Cell Survival / drug effects Cell Transformation, Neoplastic / chemically induced* Epithelial Cells / drug effects* Male Mutagens / toxicity* Pyrenes / toxicity Rats Rats, Sprague-Dawley Trachea / cytology, drug effects* Vehicle Emissions / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Benzopyrenes; 0/Mutagens; 0/Pyrenes; 0/Vehicle Emissions; 189-55-9/dibenzo(a,i)pyrene; 5522-43-0/1-nitropyrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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