Document Detail

In vitro and in vivo transformation in rat tracheal epithelial cells exposed to diesel emission particles and related compounds.
MedLine Citation:
PMID:  9600696     Owner:  NLM     Status:  MEDLINE    
The rat tracheal epithelial (RTE) cell transformation assay was performed to determine the transforming activity of diesel emission particles (DEPs) and two related compounds, 1-nitropyrene (1-NP) and dibenzo(a,i)pyrene (DBP). RTE cells were treated with these agents in vitro and in vivo. Transformed cells from foci induced by these agents were passaged over 20 times to establish immortal cell lines. Results show that (1) DEPs- and 1-NP-induced cell transformation only with the in vivo exposure (30-75 mg/kg bw DEPs and 15-60 mg/kg bw 1-NP); (2) positive dose-related responses to DBP were found with both in vitro (0.05-0.50 microg/ml) and in vivo (7.5-30 mg/kg bw) exposures; (3) the fraction of transformed foci becoming cell lines was in the order of 1-NP(25/48) > DBP(8/28) > DEPs(0/30). These results indicate that (1) DEPs, 1-NP and DBP are capable of transforming rat tracheal epithelial cells, however, the transforming activity of DEPs and -NP may be dependent on metabolic activation, and (2) transformed cells induced by DEPs have a very low probability, if any, of becoming cell lines.
M X Ensell; W Z Whong; Z C Heng; J Nath; T Ong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  412     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-06-04     Completed Date:  1998-06-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  283-91     Citation Subset:  IM    
West Virginia University, Morgantown, USA.
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MeSH Terms
Benzopyrenes / toxicity
Cell Line, Transformed
Cell Survival / drug effects
Cell Transformation, Neoplastic / chemically induced*
Epithelial Cells / drug effects*
Mutagens / toxicity*
Pyrenes / toxicity
Rats, Sprague-Dawley
Trachea / cytology,  drug effects*
Vehicle Emissions / toxicity*
Reg. No./Substance:
0/Benzopyrenes; 0/Mutagens; 0/Pyrenes; 0/Vehicle Emissions; 189-55-9/dibenzo(a,i)pyrene; 5522-43-0/1-nitropyrene

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