| In vitro and in vivo survival of bisected sheep embryos derived from frozen-thawed unsorted, and frozen-thawed sex-sorted and refrozen-thawed ram spermatozoa. | |
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MedLine Citation:
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PMID: 16185763 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ovine IVP embryos were derived from frozen-thawed unsorted and frozen-thawed sex-sorted spermatozoa that had been refrozen and thawed. The embryos were bisected and cultured in vitro, or transferred to recipient ewes to determine their survival in vitro and in vivo. Oocyte progression to the blastocyst stage was similar for unsorted (97/232, 41.8%) and sex-sorted spermatozoa (113/286, 39.5%; P > 0.05). Embryo survival in vitro post-bisection was similar for demi-embryos derived from unsorted and sex-sorted sperm, and embryos bisected at the blastocyst and expanded blastocyst stage (P > 0.05). A higher proportion of recipient ewes were pregnant at Day 63 after transfer of two intact embryos derived from unsorted (17/21, 80.9%) than two demi-embryos derived from unsorted (5/15, 33.3%) or sex-sorted spermatozoa (7/17, 41.2%). The number of fetuses per original embryo at Day 63 did not differ among groups (unsorted intact: 23/42, 54.8%; unsorted demi: 7/15, 46.7%; sex-sorted demi: 10/17, 58.8%) and twin pregnancies were observed in all groups. Embryo survival to term was high, and was not significantly different among intact (unsorted: 22/42, 52.4%) and demi-embryos (unsorted: 4/15, 26.7%; sex-sorted spermatozoa: 7/17, 41.2%; P > 0.05). Dizygotic twins (n = 6 sets) were born after the transfer of two intact embryos derived from unsorted spermatozoa, but only singleton lambs resulted from the transfer of demi-embryos. In conclusion, bisected IVP embryos successfully developed into morphologically normal lambs. However, embryo survival to term was neither increased nor decreased by embryo bisection. |
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Authors:
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Katherine M Morton; Anthony M Rowe; W M Chis Maxwell; Gareth Evans |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-26 |
Journal Detail:
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Title: Theriogenology Volume: 65 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-17 Completed Date: 2006-05-26 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
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Languages: eng Pagination: 1333-45 Citation Subset: IM |
Affiliation:
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Centre for Advanced Technologies in Animal Genetics and Reproduction (ReproGen), Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia. kmorton@vetsci.usyd.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / physiology Cell Separation Cryopreservation / methods, veterinary* Embryo Transfer / veterinary Embryo, Mammalian / physiology Female Fertilization in Vitro / methods, veterinary* Hot Temperature Male Pregnancy Semen Preservation / methods, veterinary* Sex Determination (Analysis) / veterinary* Sheep / embryology* Spermatozoa / cytology, physiology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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