Document Detail

In vitro and in vivo impairment of alpha2-adrenergic receptor-dependent antilipolysis by fatty acids in human adipose tissue.
MedLine Citation:
PMID:  11753754     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to study the influence of fatty acids on the adrenergic control of lipolysis both in vitro and in vivo. Human subcutaneous adipose tissue explants were cultured for 48 h in the presence of 100 microM bromopalmitate (BrPal), and lipolysis was measured in isolated adipocytes. In control conditions, beta-AR-dependent activation of lipolysis by epinephrine was almost undetectable, and could be fully restored by pharmacological blockade of alpha2-AR-dependent antilipolysis. After BrPal treatment, epinephrine became fully lipolytic and was no longer influenced by alpha2-AR-blockade. Radioligand binding analysis revealed that BrPal treatment led to a significant reduction in the coupling of alpha2-AR to G proteins. In parallel, a chronic and significant increase in plasma fatty acids resulting from a 4-day high-fat diet (HFD) was accompanied by an impairment of the amplifying effect of the alpha2-AR antagonist phentolamine on exercise-induced lipolysis (measured in the subcutaneous adipose tissue with the use of a microdialysis probe) normally observed after a low-fat diet. In conclusion, in vitro and in vivo studies showed that fatty acids impair alpha2-AR-dependent antilipolysis.
S Gesta; J Hejnova; M Berlan; D Daviaud; F Crampes; V Stich; P Valet; J S Saulnier-Blache
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme     Volume:  33     ISSN:  0018-5043     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2002-02-21     Revised Date:  2009-02-19    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  701-7     Citation Subset:  IM    
INSERM U317, Rangueil Hospital, Paul Sabatier University, 31403 Toulouse Cédex 4, France.
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MeSH Terms
Adipocytes / drug effects,  metabolism
Adipose Tissue / metabolism*
Adrenergic Agents / pharmacology
Biopsy, Needle
Dietary Fats / administration & dosage
Dose-Response Relationship, Drug
Epinephrine / pharmacology
Fatty Acids / pharmacology*
GTP-Binding Proteins / metabolism
Gene Expression / drug effects
Glycerol / metabolism
Idazoxan / analogs & derivatives,  metabolism,  pharmacology
Isoproterenol / pharmacology
Lipolysis / drug effects*
Middle Aged
Palmitates / pharmacology
Phentolamine / pharmacology
Receptors, Adrenergic, alpha-2 / genetics,  physiology*
Reg. No./Substance:
0/Adrenergic Agents; 0/Dietary Fats; 0/Fatty Acids; 0/Palmitates; 0/Receptors, Adrenergic, alpha-2; 102575-24-6/2-methoxyidazoxan; 50-60-2/Phentolamine; 51-43-4/Epinephrine; 56-81-5/Glycerol; 7683-59-2/Isoproterenol; 79944-58-4/Idazoxan; EC 3.6.1.-/GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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