| In vitro and in vivo haemolytic studies of tentacle-only extract from jellyfish Cyanea capillata. | |
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MedLine Citation:
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PMID: 20149860 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To approach the real haemolytic process of jellyfish toxins, both in vitro and in vivo haemolysis of tentacle-only extract (TOE) from jellyfish Cyanea capillata has been studied. Dose-response curves of the haemolytic activity of TOE in vitro were sigmoid shaped in both erythrocyte suspension and diluted whole blood, with the former more sensitive to TOE. The in vivo haemolysis increased sharply in the first 10 min and was followed by a gradual increase in the following 3h, with increasing blood potassium and lactic acid accordingly. SC5b-9 complexes were significantly up-regulated in vitro, but not in vivo. These results showed that the haemolysis of TOE in diluted whole blood and in vivo is not totally consistent with that in the erythrocyte suspension, and blood plasma might play a protective role against haemolysis. Thus we suggested that erythrocyte suspension can be used to test the damage of toxin on erythrocyte membrane, while the diluted whole blood may be more suitable to test the haemolysis of toxins. |
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Authors:
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Liang Xiao; Jing Zhang; Qian-qian Wang; Qian He; Si-hua Liu; Yue Li; Li-ming Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-10 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 24 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1203-7 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / drug effects Cnidarian Venoms / toxicity* Dose-Response Relationship, Drug Erythrocytes / drug effects, physiology, ultrastructure Hemolytic Agents / toxicity* Lactic Acid / blood Male Osmotic Fragility / drug effects Potassium / blood Rats Rats, Sprague-Dawley Scyphozoa* Toxicity Tests |
| Chemical | |
Reg. No./Substance:
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0/Cnidarian Venoms; 0/Hemolytic Agents; 50-21-5/Lactic Acid; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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