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In vitro and in vivo growth characteristics of a new ascites-type neuroblastoma cell from mouse C1300 neuroblastoma.
MedLine Citation:
PMID:  20492895     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A clonal ascited type cell, NAs-1, was obtained in culture from a mouse neuroblastoma C1300. The cells were adapted to anchorage-independently grow in the flask by the in vitro-in vivo alternate passage technique, and retained the ability of growing and producing ascites fluid when intraperitoneally injected into mice. Although the majority of growing cells in culture medium showed a small and round cell shape without any neuronal process, occasionally non-specific attachment onto the flask surface was observed, but devoid of the extrusion of processes. Karyotype analysis showed a homogeneous chromosome number, 40, with a marker chromosome [t(13:16)] and a minichromosome. Catecholamines, norepinephrine and dopamine, were found in the cell extracts and the contents of dopamine was particularly high as shown in another catecholaminergic neuroblastoma cell, N1E-115. Neuron specific enolase (?-subunit) was also detected. The treatment of the cells by dibutyryl cyclic AMP, prostaglandin E(1), or BL191 (phosphodiesterase inhibitor) induced the biochemical differentiation in terms of catecholamine and cyclic AMP contents, but failed to promote typical morphological differentiations including the extension of process or the significant promotion of adherence onto the flask surface.
Authors:
T Kato; I Horiuchi; H Kato; S Sasaki; H Tsunooka; A Masaoka; K Okumura-Noji; R Tanaka; H Fukami; K Kano-Tanaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemistry international     Volume:  7     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  1985  
Date Detail:
Created Date:  2010-05-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  29-35     Citation Subset:  -    
Affiliation:
Departments of Biochemistry and Surgery, Nagoya City University, Medical School, Mizuho-Ku, Nagoya 467, Japan.
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