Document Detail


In vitro and in vivo evaluation of lyophilized bioprosthetic valve.
MedLine Citation:
PMID:  21595717     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Freeze-drying of biological tissues allows for dry storage and gamma ray sterilization, which may improve their use as a medical prosthesis. The objective of this study was to evaluate the rehydration characteristics and hydrodynamic performance of prosthetic valves before and after lyophilization. Two size 23 bovine pericardium aortic valve prostheses from different manufacturers were evaluated in a Shelhigh (Union, NJ, USA) pulse duplicator (80 ppm, 5 L/min) before and after lyophilization. Flow and transvalvular pressure gradient were registered in vitro and in vivo, and images of opening and closing of the prosthesis were obtained in the pulse duplicator in a digital camera. Rehydration was evaluated by comparison of dry valve weight with valve weight after 15 min, and 1, 24, 48, and 72 h in saline solution, inside the pulse duplicator. In vivo performance was assessed by surgical implantation in Santa Inês young male sheep in the pulmonary position after 30 min rehydration with 0.9% saline. Transvalvular pressure gradient and flow measurements were obtained immediately after implantation and 3 months after surgery when valves were explanted. Captured images showed a change in the profile opening and closing of valve prosthesis after lyophilization. The gradient measured (in vitro) in two valves was 17.08 ± 0.57 and 18.76 ±  0.70 mm Hg before lyophilization, and 34.24 ± 0.59 and 30.40 ± 0.97 mm Hg after lyophilization. Rehydration of both lyophilized valves was approximately 82%. Drying changed the profile of the opening and closing of valve prostheses, and increased on average by 83% the gradient in vitro tests. The result of the in vivo tests suggests maintaining pressure levels of the animal with the lyophilized prostheses within acceptable levels.
Authors:
Marina J S Maizato; Sérgio A Hayashida; Fabio P Taniguchi; Olga Z Higa; Ronaldo N M Pitombo; Idágene A Cestari; Adolfo A Leirner; Noedir A G Stolf
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Artificial organs     Volume:  35     ISSN:  1525-1594     ISO Abbreviation:  Artif Organs     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  490-6     Citation Subset:  IM    
Copyright Information:
© 2011, Copyright the Authors. Artificial Organs © 2011, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
Affiliation:
Bioengineering Division, Heart Institute (InCor), University of São Paulo Medical School Biotechnology Center, Energy and Nuclear Research Institute, IPEN-CNEN/SP Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo Cardiothoracic Surgery Division, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
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