Document Detail


In vitro and in vivo characterization of putative porcine embryonic stem cells.
MedLine Citation:
PMID:  20677936     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
We have developed a new method for the isolation of porcine embryonic stem cells (ESCs) from in vivo-derived and in vitro-produced embryos. Here we describe the isolation and characterization of several ESC lines established using this method. Cells from these lines were passaged up to 14 times, during which they were repeatedly cryopreserved. During this time, ESCs maintained their morphology and continued to express Oct 4, Nanog, and SSEA1. These cells formed embryoid bodies in suspension culture, and could be directed to differentiate into various lineages representative of all three germ layers in vitro. When injected into blastocysts these cells localized in the inner cell mass of blastocysts. To examine their pluripotency further, cells were injected into host blastocysts and transferred to recipient animals. Of the six transfers undertaken, one recipient became pregnant and gave birth to a litter of one male and three female piglets. Microsatellite analysis of DNA extracted from the tail tissue of these piglets indicated that two female piglets were chimaeric.
Authors:
Ivan Vassiliev; Svetlana Vassilieva; Luke F S Beebe; Sharon J Harrison; Stephen M McIlfatrick; Mark B Nottle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular reprogramming     Volume:  12     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-08-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  223-30     Citation Subset:  IM    
Affiliation:
Reproductive Biotechnology Group, Discipline of Obstetrics and Gynaecology and Centre for Stem Cell Research, University of Adelaide , Adelaide 5005, Australia .
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